Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA
Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA
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DOI：
10.1016/j.jmb.2008.11.035
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发表时间：
2009-01-23
影响因子：
5.6
通讯作者：
Humphries, Steve E.
中科院分区：
文献类型：
--
作者：
Smith, Andrew J. P.;Humphries, Steve E.
我们描述了一种低成本的高通量技术来表征核蛋白dna结合相互作用。这项技术，被称为多路竞争对手电泳迁移转移分析，使用一系列多路寡核苷酸DNA共识竞争对手，结合标准的电泳迁移转移分析程序，有效地表征DNA结合蛋白。我们展示了该方法的实用性，以鉴定先前未报道的肝细胞核因子-3位点，该位点产生于脂蛋白脂肪酶基因的内含子8中，是通过共阴体单核苷酸多态性（rs327）产生的。(C) 2008 Elsevier Ltd版权所有。
We describe a low-cost high-throughput technique to characterize nuclear protein DNA-binding interactions. This technique, known as Multiplexed Competitor Electrophoretic Mobility Shift Assay, uses a series of multiplexed oligonucleotide DNA consensus competitors, in combination with a standard electrophoretic mobility shift assay procedure, to efficiently characterize DNA-binding proteins. We show utility for the method to identify a previously unreported hepatocyte nuclear factor-3 site created in intron 8 of the lipoprotein lipase gene by a con-anon single-nucleotide polymorphism (rs327). (C) 2008 Elsevier Ltd. All rights reserved.