The regulation of adipogenesis through GPR120
The regulation of adipogenesis through GPR120
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DOI：
10.1016/j.bbrc.2007.01.028
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发表时间：
2007-03-09
影响因子：
3.1
通讯作者：
Roh, Sang-Gun
中科院分区：
文献类型：
--
作者：
Gotoh, Chizu;Hong, Yeon-Hee;Roh, Sang-Gun
最近，已经发现长链脂肪酸激活G蛋白偶联受体（GPRs），GPR 120和GPR 40。然而，迄今为止，还没有关于这些GPR在脂肪组织发育和脂肪细胞分化中可能的生理作用的报道。GPR 120 mRNA在四种不同的脂肪组织中高度表达，并且在喂食高脂肪饮食的小鼠的脂肪组织中mRNA的量升高。然而，在任何脂肪组织中均未检测到GPR 40 mRNA。GPR 120 mRNA在脂肪细胞中的表达高于基质-血管（S-V）细胞。GPR 120 mRNA在3 T3-L1细胞脂肪分化过程中表达增加。在人脂肪组织、人前脂肪细胞和培养的脂肪细胞中观察到类似的结果。此外，使用小干扰RNA（siRNA）下调GPR 120表达导致脂肪细胞分化的抑制。我们的研究结果表明，GPR 120调节脂肪形成过程，如脂肪细胞的发育和分化。(c)2007爱思唯尔公司All rights reserved.
Recently, it has been found that long-chain fatty acids activate the G protein-coupled receptors (GPRs), GPR120 and GPR40. However, there have been no reports to date on the possible physiological roles of these GPRs in adipose tissue development and adipocyte differentiation. GPR120 mRNA was highly expressed in the four different adipose tissues, and the amount of mRNA was elevated in adipose tissues of mice fed a high fat diet. However, GPR40 mRNA was not detected in any of the adipose tissues. The expression of GPR120 mRNA was higher in adipocytes compared to stromal-vascular (S-V) cells. The level of GPR120 mRNA increased during adipocyte differentiation in 3T3-L1 cells. Similar results were observed in human adipose tissue, human preadipocytes, and cultured adipocytes. Moreover, use of a small interference RNA (siRNA) to down-regulate GPR120 expression resulted in inhibition of adipocyte differentiation. Our results suggest that GPR120 regulates adipogenic processes such as adipocyte development and differentiation. (c) 2007 Elsevier Inc. All rights reserved.