CARRIER DETECTION OF OVINE HEMOPHILIA-A USING AN RFLP MARKER, AND MAPPING OF THE FACTOR-VIII GENE ON THE OVINE X-CHROMOSOME
CARRIER DETECTION OF OVINE HEMOPHILIA-A USING AN RFLP MARKER, AND MAPPING OF THE FACTOR-VIII GENE ON THE OVINE X-CHROMOSOME
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DOI：
10.1093/oxfordjournals.jhered.a111503
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发表时间：
1994-11-01
影响因子：
3.1
通讯作者：
PLISKA, V
中科院分区：
文献类型：
--
作者：
BACKFISCH, W;NEUENSCHWANDER, S;PLISKA, V
Ovine hemophilia A is an X-linked recessive bleeding disorder. For diagnostic purposes, restriction fragment length polymorphism (RFLP) analysis in the region of the factor VIII (F-VIII) gene was carried out using human F-VIII gene probes. The probe St14, known to detect a highly polymorphic region that is closely linked to the F-VIII gene in humans, hybridized nonspecifically with DNA from sheep. Searching for intragenic RFLPs, the entire 9.0-kb coding sequence of the human F-VIII was used as a probe. Using the 1.8-kb SstI/KpnI F-VIII cDNA probe for hybridization, an Mspl-RFLP with allelic bands of 5.8 kb (A1) and 4.2 kb (A2) was detected. A1 was in linkage phase with the mutated allele responsible for hemophilia A. The F-VIII locus in the sheep genome was assigned to the long arm of the X-chromosome in the region Xq24-q33, using in situ hybridization with a 3-kb human F-VIII cDNA probe to QFQ banded sheep metaphase chromosomes.
