Formation of supported lipid bilayers on porous polymeric substrates induced by hydrophobic interaction
Formation of supported lipid bilayers on porous polymeric substrates induced by hydrophobic interaction
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DOI：
10.1016/j.colsurfa.2017.11.006
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发表时间：
2018-02
期刊：
影响因子：
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通讯作者：
Wakana Miyashita;D. Saeki;H. Matsuyama
中科院分区：
文献类型：
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作者：
Wakana Miyashita;D. Saeki;H. Matsuyama
本研究提出了一种通过疏水相互作用诱导的脂质体破裂方法在柔性和多孔聚合物基底上形成支撑脂质双层（SLB）的技术。多孔聚合物基底改善了SLB的机械强度和柔性，并允许分子渗透通过SLB。三种类型的商业聚合物微滤膜，再生纤维素（RC），硝化纤维素（NC）和聚偏氟乙烯（PVDF），被用作多孔基板。首先，使用十八烷基三氯硅烷或棕榈酰氯用烷基链对多孔基材的表面进行改性。通过X射线光电子能谱（XPS）测量来评估在衬底上的烷基链的改性程度。然后将改性的基底浸入脂质体悬浮液中。通过使用光漂白后荧光恢复（FRAP）测定法评估脂质分子在基底表面上的横向扩散率来确认SLB的形成。用于用烷基链改性衬底的方法和衬底材料的类型都影响SLB的形成。当烷基链在基底上的修饰程度高时，脂质的侧向扩散系数也高，在基底上形成平面的脂质双层结构。此外，为了显示平台对包埋各种亲脂性生物分子的适用性，将链球菌溶血素O（SLO）掺入所形成的SLB中，并用荧光标记的抗体进行免疫染色。
This study presents a technique for forming a supported lipid bilayer (SLB) on flexible and porous polymeric substrates via a liposome rupture method induced by hydrophobic interaction. Porous polymeric substrates improve the mechanical strength and flexibility of SLBs and allow the molecular permeation through SLBs. Three types of commercial polymeric microfiltration membranes, regenerated cellulose (RC), nitrocellulose (NC) and polyvinylidene fluoride (PVDF), were used as porous substrates. First, the surfaces of the porous substrates were modified with alkyl chains using octadecyltrichlorosilane or palmitoyl chloride. The degree of the alkyl chains modified on the substrates was evaluated by x-ray photoelectron spectroscopy (XPS) measurement. The modified substrates were then immersed in a liposome suspension. The SLB formation was confirmed by evaluating the lateral diffusivity of lipid molecules on the substrate surfaces using a fluorescence recovery after photobleaching (FRAP) assay. Both the method used to modify the substrates with alkyl chains and the type of substrate material affected the SLB formation. When the degree of the alkyl chains modified on the substrate was high, the lateral diffusivity of lipids was also high and planar lipid bilayer structure was formed on the substrates. Furthermore, to show the applicability for platforms to immobilize various lipophilic biomolecules, streptolysin O (SLO) was incorporated into the formed SLB and was immunostained with fluorescently labeled antibodies.