Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea.
Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea.
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DOI：
10.1266/ggs.79.87
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发表时间：
2004-04
影响因子：
1.1
通讯作者：
H. Shiba;Jong-In Park;G. Suzuki;M. Matsushita;I. Nou;A. Isogai;S. Takayama;Masao Watanabe
中科院分区：
文献类型：
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作者：
H. Shiba;Jong-In Park;G. Suzuki;M. Matsushita;I. Nou;A. Isogai;S. Takayama;Masao Watanabe
芸苔属中区分自花和非自花花粉的自交不亲和性（SI）是由S位点基因调节的。在大多数 S 单倍型中，一组三个高度多态性基因：SLG、SRK 和 SP11，位于 S 基因座区域。在本研究中，我们在甘蓝的自交不亲和S15单倍型中发现了重复的SP11基因，S15-SP11a、S15-SP11b和S15-SP11b'。 RNA凝胶印迹和逆转录聚合酶链反应（RT-PCR）分析表明，在花药组织中特异性检测到两种不同大小的SP11转录物：对应于S15-SP11a的0.65-kb转录物（在某些情况下S15-SP11b的外显子1区域也被共转录），以及包含重复的三个基因S15-SP11a、1.4-kb转录物。 S15-SP11b 和 S15-SP11b' 的外显子 1 区域，所有三个区域均与基因间间隔区域相连。
Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S15-SP11b was also co-transcribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.