AN INTEGRATED GENETIC-LINKAGE MAP FOR EUCALYPTS USING RFLP, RAPD AND ISOZYME MARKERS
AN INTEGRATED GENETIC-LINKAGE MAP FOR EUCALYPTS USING RFLP, RAPD AND ISOZYME MARKERS
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DOI：
10.1007/bf00223894
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发表时间：
1995-11-01
影响因子：
5.4
通讯作者：
MORAN, GF
中科院分区：
文献类型：
--
作者：
BYRNE, M;MURRELL, JC;MORAN, GF
构建了E. nitens是在远交的三代系谱中构建的。对210个RFLP、125个RAPD和4个同工酶位点的分析表明，330个标记连锁在12个连锁群中，总长度为1462 cM（真核生物中n = 11）。第12连锁群仅由5个标记组成，当进一步定位连锁位点时，可能与另一连锁群合并。在双亲中分离的GO-显性RFLP位点被用来整合在雄性和雌性亲本中鉴定的连锁。重组频率的差异，在两个父母中观察到的一些对的基因座，和重复的序列被确定内和之间的连锁群。除连锁群10中的RFLPs和一些显示偏分离的位点外，标记在基因组中随机分布，这些标记被聚类成三个区域。该图谱中大量共显性RFLP位点的使用使其可用于E.为E. nitens和其他欧洲物种。
An integrated genetic linkage map for E. nitens was constructed in an outbred three-generation pedigree. Analysis of 210 RFLP, 125 RAPD and 4 isozyme loci resulted in 330 markers linked in 12 linkage groups covering 1462 cM (n = 11 in eucalypts). The 12th linkage group is comprised of only 5 markers and will probably coalesce with another linkage group when further linked loci are located. Go-dominant RFLP loci segregating in both parents were used to integrate linkages identified in the male and female parents. Differences in recombination frequencies in the two parents were observed for a number of pairs of loci, and duplication of sequences was identified both within and between linkage groups. The markers were distributed randomly across the genome except for the RFLPs in linkage group 10 and for some loci showing segregation distortion, which were clustered into three regions of the map. The use of a large number of co-dominant RFLP loci in this map enables it to be used in other pedigrees of E. nitens and forms a basis for the detection and location of QTL in E. nitens and other eucalypt species.