Fabrication of high-permeability and high-capacity monolith for protein chromatography
Fabrication of high-permeability and high-capacity monolith for protein chromatography
复制标题
用于蛋白质层析的高渗透性和高容量整体材料的制造
DOI：
10.1016/j.chroma.2007.06.030
复制
发表时间：
2007-09-07
影响因子：
4.1
通讯作者：
Sun, Yan
中科院分区：
文献类型：
--
作者：
Du, Kai-Feng;Yang, Dong;Sun, Yan
提出了一种制造大孔聚（甲基丙烯酸缩水甘油酯-二甲基丙烯酸乙二醇酯）整体材料的新方法。该方法涉及使用硫酸钠颗粒和有机溶剂作为共致孔剂。与仅使用有机溶剂作为致孔剂的传统整体柱[ML-(1-3)]相比，改进的整体柱[MLS-(1-3)]不仅表现出更高的柱效和对蛋白质（牛血清白蛋白，BSA）的动态结合能力（DBC），而且还具有更高的柱渗透性和更低的背压。认为固体颗粒引入的超孔对于整体材料性能的提高起到了重要作用。此外，聚(甲基丙烯酸缩水甘油酯-二乙胺)触手被接枝到MLS-3整体材料的孔表面上。这进一步将 BSA 的 DBC 增加到 74.7 mg/ml，大约比没有接枝触手的整体高出三倍。这种接枝并没有明显降低柱的渗透性，因此为高性能制备型蛋白质色谱制备了一种具有高柱渗透性和结合能力的新型整体材料。 (C) 2007 Elsevier B.V. 保留所有权利。1
A novel approach for the fabrication of macroporous poly(glycidyl methacrylate-ethylene glycol dimethacrylate) monolith is presented. The method involved the use of sodium sulfate granules and organic solvents as co-porogens. Compared with the conventional monoliths [ML-(1-3)] using organic solvents only as a porogen, the improved monoliths [MLS-(1-3)] showed not only higher column efficiency and dynamic binding capacity (DBC) for protein (bovine serum albumin, BSA), but also higher column permeability and lower back pressure. It is considered that the superpores introduced by the solid granules played an important role for the improvement of the monolith performance. Moreover, poly(glycidyl methacrylate-diethylamine) tentacles were grafted onto the pore surface of MLS-3 monolith. This has further increased the DBC of BSA to 74.7 mg/ml, about three times higher than that of the monoliths without the grafted tentacles. This grafting does not obviously decrease the column permeability, so a new monolith of high column permeability and binding capacity has been produced for high-performance preparative protein chromatography. (C) 2007 Elsevier B.V. All rights reserved.1