Permeabilities and stabilities of large dihexadecylphosphate and dioctadecyldimethylammonium chloride vesicles
Permeabilities and stabilities of large dihexadecylphosphate and dioctadecyldimethylammonium chloride vesicles
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大二十六烷基磷酸盐和双十八烷基二甲基氯化铵囊泡的渗透性和稳定性
DOI：
10.1016/0021-9797(84)90449-1
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发表时间：
1984
影响因子：
9.9
通讯作者：
H. Chaimovich
中科院分区：
文献类型：
--
作者：
A. M. Carmona;L. Yoshida;A. Sesso;H. Chaimovich
采用氯仿蒸发和凝胶过滤的方法制备了平均外径为0.27μm的二十六烷基磷酸钠微囊。内部房水室的相对体积，估计测量[14C]蔗糖的捕获量，为13升/摩尔。DHP和双十八烷基二甲基氯化铵(DODAC)的大囊泡在室温下最稳定可达20天。相比之下，超声处理的DHP和DODAC小泡在制备后几个小时内保持稳定。DHP和DODAC大泡对大多数被测溶质都是不透水的。大的DHP囊泡对蔗糖、氯化钠或氢氧化钠的梯度反应是理想的渗透压计。合成的两亲分子和磷脂的大小泡在凝胶状态下的行为被发现是相似的。
Sodium dihexadecylphosphate (DHP) vesicles, with a mean external diameter of 0.27 μm, were obtained by chloroform vaporization followed by gel filtration. The relative volume of the internal aqueous compartment, estimated measuring entrapment of [14C] sucrose, was 13 liters/mol. DHP and dioctadecyldimethyl ammonium chloride (DODAC) large vesicles were stable up to 20 days at room temperature. In contrast, small sonicated DHP and DODAC vesicles were stable for some hours after preparation. Both DHP and DODAC large vesicles were impermeable toward most solutes tested. Large DHP vesicles responded as ideal osmometers toward gradients of sucrose, NaCl, or NaOH. The behavior of large vesicles of synthetic amphiphiles and of phospholipids was found to be analogous in the gel state.
二棕榈酰磷脂酰胆碱囊泡的融合。
影响因子：
2.9
作者：
Schullery,SE;Schmidt,CF;Felgner,P;Tillack,TW;Thompson,TE
通讯作者：
Thompson,TE