Increased serum microRNAs are closely associated with the presence of microvascular complications in type 2 diabetes mellitus.
Increased serum microRNAs are closely associated with the presence of microvascular complications in type 2 diabetes mellitus.
复制标题
血清 microRNA 增加与 2 型糖尿病微血管并发症的存在密切相关。
DOI：
10.1038/srep20032
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发表时间：
2016-02-01
影响因子：
4.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Wang C;Wan S;Yang T;Niu D;Zhang A;Yang C;Cai J;Wu J;Song J;Zhang CY;Zhang C;Wang J
循环microRNA（miRNAs）是2型糖尿病（T2 DM）的新兴生物标志物。然而，T2 DM相关微血管疾病（T2 DMC）患者血清miRNA谱的全面表征很少报道。在这项研究中，我们从184例T2 DM患者（92例有微血管并发症，92例无并发症）和92例年龄/性别匹配的对照组中获得血清样本。使用TaqMan低密度阵列（TLDA）在来自24名T2 DM患者、24名T2 DMC患者和24名对照的三个合并样品中初步分析754个miRNA的水平。随后在用于TLDA的相同样品中通过定量逆转录PCR（RT-qPCR）单独验证患者组中显著上调的miRNA，并在由68名T2 DM患者、68名T2 DMC患者和68名对照组成的另一个更大的队列中进一步证实。5种miRNA在T2 DM患者中显著上调（p < 0.05），包括miR-661、miR-571、miR-770- 5 p、miR-892 b和miR-1303。此外，有并发症的患者中五种miRNAs的水平高于无并发症的患者。回归分析显示5种miRNAs与微血管并发症显著相关（p < 0.05）。在我们的研究中确定的五种血清miRNAs有可能作为T2 DM相关微血管并发症的辅助生物标志物和新的风险因素。
Circulating microRNAs (miRNAs) are emerging biomarkers for type 2 diabetes mellitus (T2DM). However, a comprehensive characterization of the serum miRNA profile in patients with T2DM-associated microvascular disease (T2DMC) has rarely been reported. In this study, we obtained serum samples from 184 T2DM patients (92 with microvascular complications and 92 free of complications) and 92 age/gender-matched controls. The levels of 754 miRNAs were initially analyzed using a TaqMan Low Density Array (TLDA) in three pooled samples from 24 T2DM patients, 24 T2DMC patients and 24 controls. Markedly upregulated miRNAs in the patients’ groups were subsequently validated individually by quantitative reverse-transcription PCR (RT-qPCR) in the same samples used for TLDA and further confirmed in another larger cohort consisting of 68 patients with T2DM, 68 patients with T2DMC and 68 controls. Five miRNAs were significantly upregulated in T2DM patients (p < 0.05) including miR-661, miR-571, miR-770-5p, miR-892b and miR-1303. Moreover, the levels of the five miRNAs were higher in patients with complications than in those without complications. Regression analyses revealed the five miRNAs were significantly correlated with microvascular complications (p < 0.05). The five serum miRNAs identified in our study hold potential as auxiliary biomarkers and novel risk factors for T2DM-associated microvascular complications.