Generation of branching ureteric bud tissues from human pluripotent stem cells
Generation of branching ureteric bud tissues from human pluripotent stem cells
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DOI：
10.1016/j.bbrc.2017.11.105
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Osafune, Kenji
中科院分区：
文献类型：
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作者：
Mae, Shin-Ichi;Ryosaka, Makoto;Osafune, Kenji
肾脏再生研究的最新进展值得注意。然而，输尿管芽（UB），胚胎肾祖细胞，从人多能干细胞（hPSC）的选择性和强大的分化仍有待建立。本研究旨在建立一种稳健的诱导方法，用于从hPSC分支UB组织，以创建肾脏疾病模型。在这里，我们发现前中间中胚层（IM）从前原条分化，这使我们能够成功地开发出一种有效的二维分化方法的hPSC到沃尔夫管（WD）细胞。我们还建立了一个简化的程序，以产生三维WD上皮结构，可以形成分支UB组织。该系统可能有助于基于hPSC的再生疗法和用于肾脏和下尿路中出现的难治性疾病的疾病模型。(C)2017爱思唯尔公司All rights reserved.
Recent progress in kidney regeneration research is noteworthy. However, the selective and robust differentiation of the ureteric bud (UB), an embryonic renal progenitor, from human pluripotent stem cells (hPSCs) remains to be established. The present study aimed to establish a robust induction method for branching UB tissue from hPSCs towards the creation of renal disease models. Here, we found that anterior intermediate mesoderm (IM) differentiates from anterior primitive streak, which allowed us to successfully develop an efficient two-dimensional differentiation method of hPSCs into Wolffian duct (WD) cells. We also established a simplified procedure to generate three-dimensional WD epithelial structures that can form branching UB tissues. This system may contribute to hPSC-based regenerative therapies and disease models for intractable disorders arising in the kidney and lower urinary tract. (C) 2017 Elsevier Inc. All rights reserved.