Resistance from agar medium impacts the helical growth of Arabidopsis primary roots
Resistance from agar medium impacts the helical growth of Arabidopsis primary roots
复制标题
琼脂培养基的抗性影响拟南芥初根的螺旋生长
DOI：
10.1016/j.jmbbm.2018.05.018
复制
发表时间：
2018-09-01
影响因子：
3.9
通讯作者：
Hao, Shilei
中科院分区：
文献类型：
--
作者：
Yan, Jie;Wang, Bochu;Hao, Shilei
琼脂广泛用于根生长的研究，因为它可以以不同浓度混合以影响机械阻抗。在高浓度（1.2-1.5%）下，拟南芥根的生长被发现是波浪形的，但很少有研究基于对机械行为的定量理解来探索这种行为。为此，制备了浓度范围为0.5%至1.2%的琼脂培养基，以在根穿透期间产生梯度阻力，并测试了杨氏模量和弹性模量计阻力。然后将拟南芥根培养在具有梯度硬度的这些琼脂培养基中。结果表明，随着琼脂培养基浓度的增加，弹性模量呈线性增加。拟南芥初生根在0.5%~ 1.0%的琼脂培养基中最易形成螺旋状。随着琼脂硬度的增加，螺旋根的比例和螺旋直径逐渐下降，根长和生长素的分布也发生变化。我们证明了螺旋变形的大小随着琼脂刚度的减小而减小，正如基于生长诱导的机械屈曲的组合的理论分析所预期的那样。综上所述，琼脂培养基的阻力影响了螺旋根的特性，螺旋根的生长是由生长力驱动的。生长力和外部机械力对拟南芥根系表型的影响。
Agar is widely used in studies of root growth since it can be mixed at different concentrations to impact mechanical impedance. At high concentrations (1.2-1.5%), growth of Arabidopsis roots has been found to be wavy, but little research has explored this behavior based on a quantitative understanding of mechanical behavior. To this end, agar media with concentration ranging from 0.5% to 1.2% were prepared to produce gradient resistance during root penetration, and Young's moduli and penetrometer resistance were tested. Arabidopsis roots were then cultivated in these agar media with gradient stiffness. The result showed that Young's modulus increased linearly with the increase of concentration of agar media. For Arabidopsis primary roots, it was preferred to develop a helical pattern in agar media with concentration from 0.5% to 1.0%. As stiffness of agar increased, the percentage of helical roots and helix diameters in each agar medium declined; root lengths and auxin distributions showed variety. We demonstrate that the size of helical deformation decreases with agar stiffness as expected by theoretical analysis based on a combination of growth-induced mechanical buckling. In conclusion, the resistance from agar media impacts the properties of root helix, and helical roots growth is driven by growth force. Growth force and external mechanical forces contribute to root phenotypes in Arabidopsis.