Measurement of lipid transfer protein in 88 apple cultivars
Measurement of lipid transfer protein in 88 apple cultivars
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DOI：
10.1159/000112499
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发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Zuidmeer, Laurian
中科院分区：
文献类型：
--
作者：
Sancho, Ana I.;van Ree, Ronald;Zuidmeer, Laurian
背景：水果是成人食物过敏的主要原因。脂质转移蛋白（LTP）与严重的水果过敏反应有关，但对不同品种中LTP的含量知之甚少。目的：测定各种苹果品种的LTP水平。研究方法：采用三种不同的免疫测定法：竞争性ELISA（cELISA）、夹心ELISA（sELI-SA）和RAST抑制（RI），测定了生长在意大利的53个品种和生长在荷兰的35个品种的苹果中的LTP。选择的品种进行了评估，使用嗜碱性组胺释放试验（BHR），皮肤点刺试验（SPT）和双盲，安慰剂对照的食物挑战（DBPCFC）。结果如下：用三种免疫测定法测量的LTP水平显著相关，如Pearson相关性所判断的（0.61 < Rp < 0.65; p < 0.0001），但在实际量方面不同：3.4-253.2（sELISA）、2.7-120.2（cELISA）和0.4-47.3 μ g/g组织（RI）。在品种之间，LTP滴度在大约两个对数范围内变化。试点在体外和体内生物测试（BHR，SPT和DBPCFC）与选定的品种支持观察到的LTP水平的差异。结论：苹果品种之间LTP水平存在约100倍的差异。观察到的最低LTP水平是否值得指定为低过敏原，需要通过口服挑战进行更广泛的确认。确定LTP水平的品种变异为种植者和消费者提供了重要信息。与早期报道的相同品种中的Mal d 1水平相比，表明两种过敏原并不总是一致的。版权所有（C）2007 S. Karger AG，巴塞尔。
Background: Fruits are a major cause of food allergy in adults. Lipid transfer proteins (LTP) are implicated in severe allergic reactions to fruits, but little is known about LTP content in different cultivars. Objective: Determination of the levels of LTP in a wide range of apple cultivars. Methods: LTP was measured in apples from 53 cultivars grown in Italy and 35 grown in The Netherlands, using three different immunoassays: a competitive ELISA (cELISA), a sandwich ELISA (sELI-SA) and a RAST inhibition (RI). Selected cultivars were evaluated using the basophil histamine release test (BHR), skin prick test (SPT) and double-blind, placebo-controlled food challenge (DBPCFC). Results: LTP levels measured with the three immunoassays were significantly correlated, as judged by Pearson's correlation (0.61 < Rp < 0.65; p < 0.0001), but differed with respect to the actual quantities: 3.4-253.2 (sELISA), 2.7-120.2 (cELISA) and 0.4-47.3 mu g/g tissue (RI). Between cultivars, LTP titers varied over about a two-log range. Pilot in vitro and in vivo biological testing (BHR, SPT and DBPCFC) with selected cultivars supported the observed differences in LTP levels. Conclusions: Around 100-fold differences in LTP levels exist between apple cultivars. Whether the lowest observed levels of LTP warrant designation as hypo-allergenic requires more extensive confirmation by oral challenges. Determination of cultivar variation in LTP levels provides important information for growers and consumers. Comparison to earlier reported Mal d 1 levels in the same cultivars reveals that a designation as low allergenic does not always coincide for both allergens. Copyright (C) 2007 S. Karger AG, Basel.