Reusable polymer film chemosensor for ratiometric fluorescence sensing in aqueous media
Reusable polymer film chemosensor for ratiometric fluorescence sensing in aqueous media
复制标题
可重复使用的聚合物薄膜化学传感器，用于水介质中的比率荧光传感
DOI：
10.1016/j.snb.2009.12.054
复制
发表时间：
2010-03-04
影响因子：
8.4
通讯作者：
Zeng, Fang
中科院分区：
文献类型：
--
作者：
Ma, Boling;Wu, Shuizhu;Zeng, Fang
报道了一种构建聚合物薄膜传感器的新策略。首先，合成基于荧光共振能量转移（FRET）的二极体作为传感探针，利用螺旋内酰胺-罗丹明衍生物（SRhB）作为Fe3+离子的离子识别元件，通过Fe3+诱导的SRhB开环反应生成长波长罗丹明B荧光团作为能量受体，选择丹酰段作为给体，其发射光谱与SRhB开环态的吸收光谱重叠。然后将荧光二偶体共价连接到PVA基质中，形成可在水介质中用于Fe3+离子的比例荧光传感膜。这种可重复使用的独立薄膜可以像使用试纸一样方便地在水介质中实现实时传感。这种方法的识别荧光团不仅存在于薄膜表面，而且遍布整个薄膜，并且pva基薄膜可以在水中膨胀形成水凝胶，从而具有更宽的检测范围和明显的视觉观察。由于该聚合物薄膜具有低毒和生物相容性，因此可用于环境应用以及食品工业。(C) 2009 Elsevier B.V.版权所有
A novel strategy for building a polymeric film sensor was reported. First, a florescence resonance energy transfer (FRET)-based dyad was synthesized as the sensing probe, of which a spirolactam-rhodamine derivative (SRhB) was utilized as the ion-recognition element for Fe3+ ions, and a ring-opening reaction of SRhB induced by Fe3+ generates the long-wavelength rhodamine B fluorophore which can act as the energy acceptor: and dansyl moiety was chosen as the donor, whose emission spectrum overlaps with the absorption spectrum of the open-ring state of SRhB. The fluorescent dyad was then covalently linked into the PVA matrix, forming a ratiometric fluorescent sensing film usable in aqueous media for Fe3+ ions. This reusable and free-standing film can be utilized conveniently to achieve real-time sensing in aqueous medium just like using a test paper. For this approach, the recognition fluorophores reside not only on the film surface, but throughout the whole film, and the PVA-based film can swell and form into the hydrogel in water, thus allowing for wider detection range as well as obvious visual observation. This film may be used in environment applications as well as in food industry due to the low-toxic and biocompatible polymer film. (C) 2009 Elsevier B.V. All rights reserved.