Effect of silicon oxidation on long-term cell selectivity of cell-patterned Au/SiO2 platforms
Effect of silicon oxidation on long-term cell selectivity of cell-patterned Au/SiO2 platforms
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DOI：
10.1021/ja055473q
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发表时间：
2006-02-01
影响因子：
15
通讯作者：
Zhang, MQ
中科院分区：
文献类型：
--
作者：
Veiseh, M;Zhang, MQ
硅平台上的细胞图案化是开发集成的基于细胞的生物传感装置的基础，长期的细胞选择性和生物稳定性仍然是一个主要挑战。我们报告了一个基于硅的平台的发展，在一个金属绝缘体格式能够产生长期的细胞选择性的统一和生物稳定的细胞模式。用金电极阵列图案化的基底被表面工程化，使得电极用纤连蛋白活化以介导细胞附着，并且氧化硅背景用PEG钝化以抵抗蛋白质吸附和细胞粘附。三种类型的氧化物表面，即，制备天然氧化物、干热生长氧化物和湿热生长氧化物，以说明表面的氧化物状态对长期电池选择性的影响。结果表明，随着时间的推移，细胞的选择性显着不同的三个图案化的平台和最好的细胞选择性被发现在干燥的氧化物表面长达10天。表面分析结果表明，细胞选择性的这种增强可能与干燥氧化物表面上存在额外的、更活跃的氧化物状态有关，该氧化物状态支持PEG膜的稳定性并有效地抑制细胞粘附。该研究为开发稳定均匀的细胞图案化表面提供了一种新的策略，该策略适用于固定硅烷基化学品以制备生物稳定界面。
Cellular patterning on silicon platforms is the basis for development of integrated cell-based biosensing devices, for which long-term cell selectivity and biostability remain a major challenge. We report the development of a silicon-based platform in a metal-insulator format capable of producing uniform and biostable cell patterns with long-term cell selectivity. Substrates patterned with arrays of gold electrodes were surface-engineered such that the electrodes were activated with fibronectin to mediate cell attachment and the silicon oxide background was passivated with PEG to resist protein adsorption and cell adhesion. Three types of oxide surfaces, i.e., native oxide, dry thermally grown oxide, and wet thermally grown oxide, were produced to illustrate the effect of oxide state of the surface on long-term cell selectivity. Results indicated that the cell selectivity over time differed dramatically among three patterned platforms and the best cell selectivity was found on the dry oxide surface for up to 10 days. Surface analysis results suggested that this enhancement in cell selectivity may be related to the presence of additional, more active oxide states on the dry oxide surface supporting the stability of PEG films and effectively suppressing the cell adhesion. This research offers a new strategy for development of stable and uniform cell-patterned surfaces, which is versatile for immobilization of silane-based chemicals for preparation of biostable interfaces.