A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.
复制标题
DOI：
10.3389/fmicb.2017.00108
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Ricchi M
中科院分区：
文献类型：
--
作者：
Kralik P;Ricchi M
真实的时间PCR（quantitative PCR，qPCR）现在是临床和兽医诊断以及食品安全领域中用于不同微生物剂的检测、定量和分型的成熟方法。虽然PCR的概念相对简单，但qPCR中有一些特定的问题是该技术的开发人员和用户必须牢记的。这些问题包括使用正确的术语和定义、对PCR原理的理解、数据解释和呈现的困难、qPCR在微生物诊断学不同领域的局限性以及对描述qPCR性能很重要的参数。在这篇综述中，我们无意描述qPCR设计、优化和验证的每一个方面;但是，我们希望这篇基本指南将有助于引导qPCR的初学者和用户使用这种强大的技术。
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.