Nonionic surfactant-capped gold nanoparticles as postcolumn reagents for high-performance liquid chromatography assay of low-molecular-mass biothiols
Nonionic surfactant-capped gold nanoparticles as postcolumn reagents for high-performance liquid chromatography assay of low-molecular-mass biothiols
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DOI：
10.1016/j.chroma.2007.07.045
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发表时间：
2007-09-07
影响因子：
4.1
通讯作者：
Yam, Vivian Wing-Wah
中科院分区：
文献类型：
--
作者：
Lu, Chao;Zu, Yanbing;Yam, Vivian Wing-Wah
Gold nanoparticles (GNPs) have been stabilized with nonionic surfactant ligands, i.e., Brij (R) 35, and their aggregation could be induced rapidly and selectively by biologically active low-molecular-mass thiols including sulphydryl-containing amino acids (cysteine and homocysteine) and small peptides (glutathione, cysteinylglycine, and glutamylcysteine). A new postcolumn detection method has been developed for high-performance liquid chromatography (HPLC) assay of these small biothiols based on the analyte-induced aggregation of the GNPs. Compared with conventional thiol-reactive probes, the GNP colloids are easier to prepare, much more stable in aqueous solution over a wide pH range and at ambient temperature, and exhibit relatively high selectivity toward small biothiols. The analysis of human urine samples demonstrated that the proposed method is promising in HPLC assay of the small thiol molecules in biological fluids. (C) 2007 Elsevier B.V. All rights reserved.
