The CYP1D subfamily of genes in mammals and other vertebrates
The CYP1D subfamily of genes in mammals and other vertebrates
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DOI：
10.1007/s00335-010-9263-9
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发表时间：
2010-06-01
期刊：
影响因子：
2.5
通讯作者：
Ishizuka, Mayumi
中科院分区：
文献类型：
--
作者：
Kawai, Yusuke K.;Ikenaka, Yoshinori;Ishizuka, Mayumi
细胞色素P450家族1（CYP 1）的成员参与许多环境污染物和植物化学物质如多环芳烃（PAH）、芳香胺和黄酮类化合物的解毒和生物活化。脊椎动物CYP 1基因包括四个亚家族：CYP 1A、CYP 1B、CYP 1C和CYP 1D。最近，CYP1D基因在鱼类中被发现，随后在鸭嘴兽中被发现。这些发现表明所有脊椎动物都有一个功能性CYP1D亚家族的可能性。然而，没有关于哺乳动物CYP1D基因的信息。在这项研究中，我们研究了CYP1D基因在哺乳动物和其他脊椎动物中的基因组定位。我们还进行了系统发育分析，并计算CYP1D序列的同一性和相似性。通过对CYP1D基因的同线性和系统发育分析，揭示了CYP1基因家族的进化历史。结果表明，CYP1D成为一个无功能的假基因在人类和牛的物种，但其他一些哺乳动物具有功能的CYP1D基因。对CYP1D基因启动子区进行了分析。与其他CYP1亚型不同，在CYP1D基因上游发现了一些异生素反应元件（XRE）样序列。对哺乳动物CYP1Ds的分析也为CYP1基因和芳烃受体之间的关系提供了新的见解。
Members of the cytochrome P450 family 1 (CYP1s) are involved in the detoxification and bioactivation of numerous environmental pollutants and phyto-chemicals such as polycyclic aromatic hydrocarbons (PAHs), aromatic amines, and flavonoids. The vertebrate CYP1 gene comprises four subfamilies: CYP1A, CYP1B, CYP1C, and CYP1D. Recently, the CYP1D gene was identified in fish, and subsequently in the platypus. These findings indicate the possibility that all vertebrates have a functional CYP1D subfamily. However, there is no information on the mammalian CYP1D gene. In this study we investigated the genomic location of CYP1D genes in mammals and other vertebrates in silico. We also performed phylogenetic analysis and calculated the identities and similarities of CYP1D sequences. The data from synteny and phylogenetic analyses of CYP1D genes demonstrated the evolutionary history of the CYP1 gene family. The results suggested that CYP1D became a nonfunctional pseudogene in human and bovine species; however, several other mammals possess functional CYP1D genes. The promoter regions of CYP1D genes were also examined. Unlike other CYP1 isoforms, few xenobiotic responsive element (XRE)-like sequences were found upstream of the CYP1D genes. Analysis of mammalian CYP1Ds also provided new insight into the relationship between CYP1 genes and the aryl hydrocarbon receptor.