Continuous-Wave Stimulated Raman Scattering (cwSRS) Microscopy.
Continuous-Wave Stimulated Raman Scattering (cwSRS) Microscopy.
复制标题
连续波受激拉曼散射 (cwSRS) 显微镜。
DOI：
10.1007/s00340-013-5405-6
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yakovlev,VladislavV
中科院分区：
文献类型：
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作者：
Meng,Zhaokai;Petrov,GeorgiI;Yakovlev,VladislavV
Stimulated Raman scattering (SRS) microscopy is a powerful tool for chemically sensitive non-invasive optical imaging. However, ultrafast laser sources, which are currently employed, are still expensive and require substantial maintenance to provide temporal overlap and spectral tuning. SRS imaging, which utilizes continuous-wave laser sources, has a major advantage, as it eliminates the cell damage due to exposure to the high-intensity light radiation, while substantially reducing the cost and complexity of the setup. As a proof-of-principle, we demonstrate microscopic imaging of dimethyl sulfoxide using two independent, commonly used lasers, a diode-pumped, intracavity doubled 532-nm laser and a He–Ne laser operating at 632.8-nm.
