Histological demonstration of mosaicism in a series of chimeric rats produced between congenic strains.
Histological demonstration of mosaicism in a series of chimeric rats produced between congenic strains.
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在同系品系之间产生的一系列嵌合大鼠中嵌合的组织学证明。
DOI：
10.1126/science.3966159
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Iannaccone,PM
中科院分区：
文献类型：
--
作者：
Weinberg,WC;Howard,JC;Iannaccone,PM
实验嵌合体的产生聚集桑椹胚从PVG大鼠不同的主要组织相容性复合体（RT 1）的同类系。将抗两种菌株的I类抗原变体的单克隆抗体直接与碘-125缀合并应用于组织切片。放射自显影可以检查大多数内部组织。PVG-RT 1acells在嵌合体红细胞群中的比例从8%到70%不等，用荧光激活流式细胞术测定。放射自显影图的数字分析表明，PVG-RT 1a细胞对嵌合体肝脏的贡献范围为34%至86%。肝脏中每种基因型的细胞斑块在几何上都很复杂，大小变化很大。胸腺，但不是脾脏，显示寡克隆发展的证据。肾上腺皮质呈放射状条纹状，提示干细胞的克隆性扩增。利用这种方法，可以通过直接的组织学可视化来测量嵌合体中的细胞分布，这在大鼠器官发生的研究中可能是有用的。
Experimental chimeras were produced by aggregating morulae from congenic strains of PVG rats differing in the major histocompatibility complex (RT1). Monoclonal antibodies against variant class I antigens of the two strains were directly conjugated to iodine-125 and applied to tissue sections. Autoradiograms allowed examination of most internal tissues. The proportion of PVG-RT1acells in the erythrocyte populations of the chimeras varied from 8 to 70 percent, as determined with fluorescence-activated flow cytometry. Digital analysis of autoradiograms demonstrated that the contribution of PVG-RT1acells to the livers of the chimeras ranged from 34 to 86 percent. Patches of cells of each genotype in the liver were geometrically complex, with large variations in size. The thymus, but not the spleen, showed evidence of oligoclonal development. The adrenal cortex revealed a radially striped pattern, suggestive of clonal expansion of stem cells. With this approach it is possible to measure cell distribution in chimeras through direct histological visualization, which may prove useful in the study of rat organogenesis.