Autoconfocal microscopy with nonlinear transmitted light detection
Autoconfocal microscopy with nonlinear transmitted light detection
复制标题
具有非线性透射光检测功能的自共焦显微镜
DOI：
10.1364/josab.21.001486
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发表时间：
2004
影响因子：
1.9
通讯作者：
J. Mertz
中科院分区：
文献类型：
--
作者：
T. Pons;J. Mertz
我们描述了一种简单而强大的透射共聚焦激光扫描显微镜技术，其中检测针孔被薄的二次谐波产生晶体所取代。该技术的优点是无需对信号进行反扫描即可实现自对准共聚焦。我们推导了仪器的点扩展函数，并量化了在浑浊板内成像时的信号退化和背景抑制。作为一个例子，我们考虑一个平板，其折射率波动表现为高斯统计。我们的模型得到了实验的证实。
We describe a simple and robust technique for transmission confocal laser scanning microscopy wherein the detection pinhole is replaced by a thin second-harmonic generation crystal. The advantage of this technique is that self-aligned confocality is achieved without the need for signal descanning. We derive the point-spread function of our instrument and quantify both signal degradation and background rejection when imaging deep within a turbid slab. As an example, we consider a slab whose index of refraction fluctuations exhibit Gaussian statistics. Our model is corroborated by experiment.