THE FORMATION OF THE NOTOCHORD IN THE AMPHIBIA
THE FORMATION OF THE NOTOCHORD IN THE AMPHIBIA
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两栖动物脊索的形成
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通讯作者：
H. D. King
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作者：
H. D. King
对普通蟾蜍（Bufo lentiginosus）和蛙（Rana palustris）脊索发育方式的研究，揭示了某些与相关类群中相同结构的形成有关的观点。沿着这条线索，人们已经做了沿着大量的工作，然而，胚胎学家对两栖类脊索的起源存在着很大的分歧。希望本文所记录的结果能有助于澄清这一问题。所用材料固定在腐蚀性醋酸（5？冰醋酸），切片在载玻片上用硼砂-胭脂红和里昂蓝的混合物染色，如先前的论文（King，II）所述。当这种染色剂用于新鲜保存的材料时，效果特别好，因为所有的细胞核都变成暗红色，外胚层和中胚层呈现深蓝色，而蛋黄细胞只呈现淡蓝色，因此很容易与其他细胞区分开来。这种清晰的组织结构非常有用，特别是在蟾蜍切片的研究中。本文件中给出的所有图纸都是在照相机的帮助下绘制的。
A study of the mode of development of the notochord in the common toad, Bufo lentiginosus, and of the frog, Rana palustris, has brought to light certain points that have a bearing on the formation of the same structure in related groups. A vast amount of work has already been done along this line, yet a wide difference of opinion exists among embryologists regarding the origin of the notochord in the Amphibia. It is hoped that the results recorded in the present paper may help to clear up this question. The material used was fixed in corrosive-acetic (5? glacial acetic acid), and the sections were stained on the slide with a mixture of borax-carmine and Lyon's blue as described in a previous paper (King, I I). This stain gives particularly good results when it is used on freshly preserved material, as then all of the nuclei become dark red, the ectoderm and mesoderm appear dark blue, while the yolk cells take but a pale blue tint and, therefore, are easily distinguished from the other cells. This sharp definition of the tissues was of great assistance, particularly in the study of the sections of Bufo. All of the drawings given in the present paper were outlined with the aid of a camera lucida.