NEURON SCHWANN-CELL INTERACTION IN BASAL LAMINA FORMATION
NEURON SCHWANN-CELL INTERACTION IN BASAL LAMINA FORMATION
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DOI：
10.1016/0012-1606(82)90190-7
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
WOOD, PM
中科院分区：
文献类型：
--
作者：
BUNGE, MB;WILLIAMS, AK;WOOD, PM
组织培养系统允许神经细胞、雪旺细胞和成纤维细胞单独或以各种组合生长，这使得研究细胞相互作用在周围神经系统发育中的作用成为可能。利用这些系统，早期发现在感觉神经节细胞和无成纤维细胞的雪旺细胞培养中，在雪旺细胞表面形成基底层。在这里，神经细胞的存在被证明是在许旺细胞质膜上形成基底层所必需的。使用不含成纤维细胞的神经细胞-雪旺细胞[大鼠]制剂。当神经细胞从3- 5周龄的培养物中取出时，雪旺细胞的基底层消失。如果将神经细胞重新加入到这样的雪旺细胞群中，雪旺细胞基层就会重新出现。从年龄较大（3-4个月）的培养物中去除神经细胞不会导致基底膜丧失；被认为在神经突变性之前未被层膜覆盖的区域仍然如此，这表明层膜存在但未被改造。如果用胰蛋白酶去除基板，它在神经元加雪旺细胞培养中被改造，但在雪旺细胞群中不被改造。雪旺细胞基底膜的形成需要神经细胞的存在，而不是持久存在。
The availability of tissue culture systems that allow the growth of nerve cells, Schwann cells and fibroblasts separately or in various combinations permits investigation of the role of cell interactions in the development of the peripheral nervous system. Using these systems it was earlier found that basal lamina is formed on the Schwann cell surface in cultures of sensory ganglion cells and Schwann cells without fibroblasts. Here, the presence of nerve cells is shown to be required for the generation of basal lamina on the Schwann cell plasmalemma. Nerve cell-Schwann cell [rat] preparations devoid of fibroblasts were used. When nerve cells are removed from 3- to 5-wk-old cultures, the basal lamina disappears from Schwann cells. If nerve cells are added back to such Schwann cell populations, Schwann cell basal lamina reappears. Removal of nerve cells from older (3-4 mo.) cultures does not lead to basal lamina loss; areas presumed not to have been coated with lamina before neurite degeneration remain so, suggesting that the lamina persists but is not reformed. If basal lamina is removed with trypsin, it is reformed in neuron plus Schwann cell cultures but not in Schwann cell populations alone. Formation but not the persistence of Schwann cell basal lamina requires the presence of nerve cells.