THE TOXIN BINDING-INHIBITION TEST AS A RELIABLE INVITRO ALTERNATIVE TO THE TOXIN NEUTRALIZATION TEST IN MICE FOR THE ESTIMATION OF TETANUS ANTITOXIN IN HUMAN-SERA
THE TOXIN BINDING-INHIBITION TEST AS A RELIABLE INVITRO ALTERNATIVE TO THE TOXIN NEUTRALIZATION TEST IN MICE FOR THE ESTIMATION OF TETANUS ANTITOXIN IN HUMAN-SERA
复制标题
DOI：
10.1016/0092-1157(88)90017-0
复制
发表时间：
1988-01-01
期刊：
影响因子：
--
通讯作者：
KREEFTENBERG, JG
中科院分区：
文献类型：
--
作者：
HENDRIKSEN, CFM;VANDERGUN, JW;KREEFTENBERG, JG
本文介绍了一种筛选人血清破伤风抗体的方法。毒素结合抑制试验（ToBI试验）基于破伤风抗体对破伤风毒素与抗毒素包被免疫测定微量滴定板结合的抑制。通过小鼠毒素中和（TN）试验、类毒素ELISA和ToBI试验，对191名具有不同疫苗接种史的健康成年人的血清样本进行了破伤风抗体滴定。在各个方面，ToBI测试被证明是最好的体外替代小鼠的TN测试。比较显示ToBI-测试和TN-测试之间的相关程度高于类毒素-ELISA和TN-测试之间的相关程度。此外，通过ToBI试验滴定低滴度时未观察到抗体含量高估。相比之下，当使用类毒素-ELISA时，观察到几个假阳性结果。它的结论是，ToBI测试是一个可靠的和精确的替代N-测试，可以在简单的实验室条件下在短时间内进行。
A method for the screening of human sera for tetanus antibodies has been developed and evaluated. The toxin binding inhibition test (ToBI-test) is based on inhibition of the binding of tetanus toxin to an antitoxin-coated immunoassay microtitre plate by tetanus antibodies. Serum samples from 191 healthy adults with different vaccination histories have been titrated for tetanus antibodies by the toxin neutralization (TN) test in mice, by toxoid-ELISA and by the ToBI-test. In every respect, the ToBI-test proved to be the best in vitro alternative to the TN-test in mice. Comparison showed a higher degree of correlation between the ToBI-test and the TN-test than between the toxoid-ELISA and the TN-test. Furthermore, no overestimation of antibody content was seen in titrating low titre by the ToBI-test. In contrast, several false positive results were seen when using the toxoid-ELISA. It is concluded that the ToBI-test is a reliable and precise alternative to the N-test and can be performed under simple laboratory conditions in a short time.