A zinc-dependent peptidase in prostatic organelles present in seminal plasma.
A zinc-dependent peptidase in prostatic organelles present in seminal plasma.
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精浆中前列腺细胞器中的锌依赖性肽酶。
DOI：
10.1016/0009-8981(82)90032-8
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
G. Rannevik
中科院分区：
文献类型：
--
作者：
C. Laurell;H. Weiber;K. Ohlsson;G. Rannevik
如果射精后不冷冻血浆，精浆电泳蛋白模式在几个小时内就会发生明显变化。主要是阴极蛋白消失。添加丝氨酸蛋白酶抑制剂没有明显影响，但丁菲咯啉显着延迟了电泳上观察到的转化。精浆中的亚细胞器含有锌离子依赖性肽酶，在 pH 7.8 时具有最佳活性。可以使用弹性蛋白酶底物n-叔丁氧基羰基-1-丙氨酸-对硝基苯酯(Boc(Ala))和琥珀酰(丙氨酸)3-对硝基苯胺(Suc(Ala)3pNA)来估计酶活性。该酶通过添加邻菲咯啉而失活，并通过添加锌离子而完全重新激活。
The electrophoretic seminal plasma protein pattern changes obviously within a few hours if the plasma is not frozen after the ejaculation. Mainly cathodal proteins disappear. Addition of serine protease inhibitors has no apparent affect, buto-phenanthroline markedly retards the conversions seen on electrophoresis. The subcellular organelles in seminal plasma are shown to contain a zinc ion dependent peptidase with optimal activity at pH 7.8. Estimations of enzyme activity may be performed using the elastase substratesn-tert butyloxycarbonyl-l-alanine-paranitrophenylester (Boc(Ala)) and succinyl(alanine)3-paranitroanilide (Suc(Ala)3pNA). The enzyme is inactivated by addition of o-phenanthroline and is completely reactivated by the addition of zinc ions.