Overexpression of an ABA-dependent grapevine bZIP transcription factor, VvABF2, enhances osmotic stress in Arabidopsis
Overexpression of an ABA-dependent grapevine bZIP transcription factor, VvABF2, enhances osmotic stress in Arabidopsis
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DOI：
10.1007/s00299-019-02389-y
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发表时间：
2019-05-01
影响因子：
6.2
通讯作者：
Cheng, Zong-Ming
中科院分区：
文献类型：
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作者：
Liu, Jinyi;Chu, Jinjin;Cheng, Zong-Ming
关键信息葡萄藤VvABF2基因的过表达可以增强拟南芥的渗透胁迫耐受性，但这完全需要阿坝信号的参与。然而，它在其他特定物种（例如葡萄藤）中的功能尚未完全表征。本研究以葡萄藤（葡萄属vinifera L.）从拟南芥中克隆了脱落酸应答元件结合因子2（VvABF 2）的同源基因，并在花椰菜花叶病毒35S启动子的控制下在拟南芥中进行了组成型表达。与野生型植物相比，VvABF2转基因拟南芥植物表现出对外源阿坝更敏感，并且表现出显著的渗透耐受性，如聚乙二醇（PEG）和干旱，但完全需要阿坝来进行信号传导。其下游基因表达测定进一步证实了这一事实。此外，对转基因植株体内活性氧（ROS）抗氧化酶（包括SOD、POD和CAT）和丙二醛（MDA）含量的测定表明，VvABF2在拟南芥中的过表达显著提高了活性氧的清除能力，从而减轻了细胞膜的损伤，这可能是ABA非依赖性的。我们的研究结果提供的证据表明，VvABF2具有类似的功能，拟南芥同源物在响应渗透胁迫，并有一个类似的祖先的功能，这个基因在ABA依赖的响应压力在葡萄藤。
Key messageOverexpression of grapevine VvABF2 gene could enhance osmotic stress tolerance in Arabidopsis thaliana but fully required for ABA signaling.AbstractThe abscisic acid (ABA)-dependent AREB/ABF-SnRK2 pathway has been demonstrated to play a pivotal role in response to osmotic stress in model plants. However, its function in other specific species, for example grapevine, has not been fully characterized. In this study, grapevine (Vitis vinifera L.) ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), a homologous gene of AREB/ABFs form Arabidopsis, was isolated and constitutively expressed in Arabidopsis under the control of the cauliflower mosaic virus 35S promoter. The VvABF2 transgenic Arabidopsis plants showed to be more sensitive to exogenous ABA compared to wild type plants and exhibited significant osmotic tolerance, like polyethylene glycol (PEG) and drought but fully required ABA for signaling. This fact was further confirmed by its downstream gene expression assays. In addition, the determination of ROS antioxidant enzymes (including SOD, POD and CAT) and the MDA of transgenic lines indicated that overexpression of VvABF2 in Arabidopsis significantly increased ROS scavenging ability and thereby reduced the cell membrane damage, which might be ABA-independent. Our results provide evidence that VvABF2 has a similar function to the Arabidopsis homolog in response to osmotic stresses, and that there is a similar ancestral function of this gene in ABA-dependent response to stresses in grapevine.