TRANSFORMATION AND GROWTH RELATED CHANGES IN LEVELS OF NUCLEAR AND CYTOPLASMIC PROTEINS ANTIGENICALLY RELATED TO MAMMALIAN BETA-GALACTOSIDE-BINDING LECTIN
TRANSFORMATION AND GROWTH RELATED CHANGES IN LEVELS OF NUCLEAR AND CYTOPLASMIC PROTEINS ANTIGENICALLY RELATED TO MAMMALIAN BETA-GALACTOSIDE-BINDING LECTIN
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DOI：
10.1016/s0006-291x(85)80215-1
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
FEIZI, T
中科院分区：
文献类型：
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作者：
CARDING, SR;THORPE, SJ;FEIZI, T
使用针对13kDa[千道尔顿]哺乳动物β-半乳糖苷结合凝集素的单克隆抗体进行的免疫荧光和免疫印迹实验表明，人淋巴细胞含有表观分子量为130、80、65和13kDa的核蛋白和细胞质蛋白，这些蛋白与凝集素抗原相关，并且其表达水平和模式与转化有关或在用有丝分裂原刺激后发生变化。这些观察结果以及骨髓细胞系 K562 也富含 130 kDa 成分的发现；由于正常供体和慢性粒细胞白血病患者的成熟粒细胞缺乏所有免疫反应形式，因此该凝集素相关蛋白家族可能是生长调节系统的组成部分，在转化和刺激的细胞中以不同方式引发。
Immunofluorescence and immunoblotting experiments, using a monoclonal antibody to the 13 kDa [kilodalton] mammalian .beta.-galactoside-binding lectin have shown that human lymphocytes contain nuclear and cytoplasmic proteins of apparent molecular masses of 130, 80, 65 and 13 kDa that are antigenically related to the lectin and whose levels and patterns of expression change in association with transformation, or after stimulation with mitogens. These observations, together with the finding that the myeloid cell line K562 is also rich in the 130 kDa component; as the mature granulocytes of normal donors and of patients with chronic myeloid leukemia are lacking in all of the immunoreactive forms, raise the possibility that this family of lectin-related proteins may be components of growth regulatory systems that are variously elicited in the transformed and stimulated cells.