The small heat shock protein cage from Methanococcus jannaschii is a versatile nanoscale platform for genetic and chemical modification
The small heat shock protein cage from Methanococcus jannaschii is a versatile nanoscale platform for genetic and chemical modification
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DOI：
10.1021/nl034786l
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发表时间：
2003-11-01
期刊：
影响因子：
10.8
通讯作者：
Douglas, T
中科院分区：
文献类型：
--
作者：
Flenniken, ML;Willits, DA;Douglas, T
大自然为我们提供了一系列反应性的纳米级平台，以蛋白质笼子结构的形式，如病毒衣壳和铁蛋白样蛋白质的笼子。蛋白质笼结构具有明确划分的外部、内部和由精确定位的化学功能组成的界面表面。在目前的工作中，我们证明了来自jannaschii甲烷球菌的小热休克蛋白（MjHsp）笼是一种新的多功能纳米级平台，其内外表面都可以进行遗传和化学修饰。野生型和遗传突变型的热休克蛋白笼显示与活化荧光素分子以特定位点的方式反应。此外，12 nm的热稳定笼可以作为铁氧化矿化的反应容器，从而形成单分散的9 nm氧化铁纳米颗粒。这些结果证明了热稳定笼作为合成软（有机）和硬（无机）材料的纳米级平台的实用性。
Nature has provided us with a range of reactive nanoscale platforms, in the form of protein cage architectures such as viral capsids and the cages of ferritin-like proteins. Protein cage architectures have clearly demarcated exterior, interior, and interface surfaces consisting of precisely located chemical functionalities. In the present work, we demonstrate that the small heat shock protein (MjHsp) cage from Methanococcus jannaschii is a new and versatile nanoscale platform whose exterior and interior surfaces are amenable to both genetic and chemical modification. Wild type and genetic mutants of the Hsp cage are shown to react with activated fluorescein molecules in a site specific manner. In addition, the 12 nm Hsp cage serves as a size constrained reaction vessel for the oxidative mineralization of iron, resulting in the formation of monodispersed 9 nm iron oxide nanoparticles. These results demonstrate the utility of the Hsp cage to serve as a nanoscale platform for the synthesis of both soft (organic) and hard (inorganic) materials.