A 3D-printed transfusion platform reveals beneficial effects of normoglycemic erythrocyte storage solutions and a novel rejuvenating solution.
A 3D-printed transfusion platform reveals beneficial effects of normoglycemic erythrocyte storage solutions and a novel rejuvenating solution.
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DOI：
10.1039/d2lc00030j
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发表时间：
2022-03-29
期刊：
影响因子：
6.1
通讯作者：
Spence, Dana M.
中科院分区：
文献类型：
--
作者：
Liu, Yueli;Hesse, Laura E.;Geiger, Morgan K.;Zinn, Kurt R.;McMahon, Timothy J.;Chen, Chengpeng;Spence, Dana M.
开发了一套3D打印分析设备，用于研究在输血医学中使用的常规和改进的存储溶液中处理的红细胞(ERY)。在输注给患者接受者之前的储存过程中，ERY经历了许多尚未完全了解的化学和物理变化。然而，这些变化被认为导致输血后并发症的增加，甚至死亡率的增加。在这里，可重复使用的流体设备(用添加剂制造技术制造)能够在引入流动的新鲜ERY之前和之后对ERY进行评估，从而在体外平台上表示体内ERY输注的成分。具体地说，用化学发光法测定了储存在常规溶液和葡萄糖修饰溶液中的ERY释放流动诱导的ATP的能力。使用3D打印支架中的一种新的膜传输方法，还确定了ERYS在整个储存期间的变形性。结果表明，高血糖条件会永久性地改变细胞的变形能力，这可能解释了ATP释放减少的原因，因为这一现象与细胞的变形能力有关。重要的是，在体外输血模型中，降低的变形性和ATP的释放是可逆的；具体地说，当储存的细胞被引入流动的健康细胞流中时，由ERY派生的ATP的释放和细胞的变形性都恢复到与未储存的细胞相似的状态。然而，在储存1-2周后，储存的有害影响是永久性的。这些结果表明，目前批准的高血糖储存溶液正在对用于输血药物的储存ERY产生不利影响，而正常血糖储存可能会减少储存损伤，特别是对于储存超过14天的细胞。
A set of 3D-printed analytical devices were developed to investigate erythrocytes (ERYs) processed in conventional and modified storage solutions used in transfusion medicine. During storage prior to transfusion into a patient recipient, ERYs undergo many chemical and physical changes that are not completely understood. However, these changes are thought to contribute to an increase in post-transfusion complications, and even an increase in mortality rates. Here, a reusable fluidic device (fabricated with additive manufacturing technologies) enabled the evaluation of ERYs prior to, and after, introduction into a stream of flowing fresh ERYs, thus representing components of an in vivo ERY transfusion on an in vitro platform. Specifically, ERYs stored in conventional and glucose-modified solutions were assayed by chemiluminescence for their ability to release flow-induced ATP. The ERYs deformability was also determined throughout the storage duration using a novel membrane transport approach housed in a 3D-printed scaffold. Results show that hyperglycemic conditions permanently alter ERY deformability, which may explain the reduced ATP release, as this phenomenon is related to cell deformability. Importantly, the reduced deformability and ATP release were reversible in an in vitro model of transfusion; specifically, when stored cells were introduced into a flowing stream of healthy cells, the ERY-derived release of ATP and cell deformability both returned to states similar to that of non-stored cells. However, after 1–2 weeks of storage, the deleterious effects of the storage were permanent. These results suggest that currently approved hyperglycemic storage solutions are having adverse effects on stored ERYs used in transfusion medicine and that normoglycemic storage may reduce the storage lesion, especially for cells stored for longer than 14 days.
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DOI：
10.1056/nejmoa1414219
发表时间：
2015-04-09
期刊：
The New England journal of medicine
影响因子：
--
作者：
Steiner ME;Ness PM;Assmann SF;Triulzi DJ;Sloan SR;Delaney M;Granger S;Bennett-Guerrero E;Blajchman MA;Scavo V;Carson JL;Levy JH;Whitman G;D'Andrea P;Pulkrabek S;Ortel TL;Bornikova L;Raife T;Puca KE;Kaufman RM;Nuttall GA;Young PP;Youssef S;Engelman R;Greilich PE;Miles R;Josephson CD;Bracey A;Cooke R;McCullough J;Hunsaker R;Uhl L;McFarland JG;Park Y;Cushing MM;Klodell CT;Karanam R;Roberts PR;Dyke C;Hod EA;Stowell CP
通讯作者：
Stowell CP
影响因子：
2.9
作者：
Roussel, Camille;Dussiot, Michael;Amireault, Pascal
通讯作者：
Amireault, Pascal
DOI：
10.1152/ajpheart.1996.271.6.h2717
发表时间：
1996-12-01
影响因子：
4.8
作者：
Sprague, RS;Ellsworth, ML;Lonigro, AJ
通讯作者：
Lonigro, AJ
影响因子：
4.6
作者：
Geiger M;Janes T;Keshavarz H;Summers S;Pinger C;Fletcher D;Zinn K;Tennakoon M;Karunarathne A;Spence D
通讯作者：
Spence D
DOI：
10.1182/asheducation-2011.1.475
发表时间：
2011
期刊：
Hematology. American Society of Hematology. Education Program
影响因子：
--
作者：
Roback JD
通讯作者：
Roback JD