The association of allergic sensitization patterns in early childhood with disease manifestations and immunological reactivity at 10 years of age.
The association of allergic sensitization patterns in early childhood with disease manifestations and immunological reactivity at 10 years of age.
复制标题
儿童早期过敏致敏模式与 10 岁时疾病表现和免疫反应性的关联。
DOI：
10.1111/cea.13406
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Sette,Alessandro
中科院分区：
文献类型：
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作者：
Schulten,Véronique;Frazier,April;Calatroni,Agustin;Kattan,Meyer;Bacharier,LeonardB;O'Connor,GeorgeT;Sandel,MeganT;Wood,RobertA;Wheatley,LisaM;Togias,Alkis;Visness,CynthiaM;Dresen,Amy;Gern,JamesE;Sette,Alessandro
对德国蜚蠊（German蜚蠊，CR）的过敏在城市环境中很常见，是儿童哮喘的重要过敏原。目的：我们假设过敏性致敏和临床疾病的演变与不同的过敏原特异性T细胞反应性模式有关。为了验证这一假设，研究人员选择了一组高风险的城市内儿童（URECA，城市环境和儿童哮喘）出生队列，根据2至10岁空气过敏原敏感性的获得情况，从三个不同的组中评估CR特异性T细胞的反应性：低特应性，最低至无敏感性（n = 26），早发型过敏致敏（n = 25）和晚发型过敏致敏（n = 25）。方法使用先前鉴定的CR来源的T细胞表位池，我们从这76名10岁时获得的血液样本中表征了过敏原特异性T细胞反应。用CR提取物体外培养两周后，用ELISPOT检测IL - 5、IFNγ和IL - 10的CR特异性产生。结果早发型特应性组的细胞反应明显高于低发型特应性组（P= 0.01），晚发型组的细胞因子产生也明显高于低发型特应性组（P= 0.06）。T细胞反应在早发和晚发队列中相似。此外，哮喘和非哮喘个体之间的T细胞反应性比较显示，在CR过敏和非过敏队列中，哮喘患者的细胞因子产生明显高于非哮喘患者（P= 0.02）。总之，本研究报告了高T细胞反应性与过敏原致敏和哮喘有关。有趣的是，早发型过敏性儿童与晚发型过敏性儿童的T细胞反应性无显著差异。
BackgroundAllergy to German cockroach (CR) is common in urban environments and is an important allergen in children with asthma.ObjectiveWe hypothesize that the evolution of allergic sensitization and clinical disease is associated with distinct patterns of allergen‐specific T cell reactivity. To test this hypothesis, a subset of high‐risk inner‐city children participating in the URECA (Urban Environment and Childhood Asthma) birth cohort were selected to evaluate CR‐specific T cell reactivity from three distinct groups based on acquisition of aeroallergen sensitivity from ages 2 to 10: low atopy with minimal to no sensitivity (n = 26), early‐onset allergic sensitization (n = 25) and late‐onset allergic sensitization (n = 25).MethodsUsing pools of previously identified CR‐derived T cell epitopes, we characterized the allergen‐specific T cell response in these 76 subjects from blood samples obtained at age 10. CR‐specific production of IL‐5, IFNγ and IL‐10 was measured by ELISPOT following two‐week in vitro culture with CR extract.ResultsT cell responses were significantly higher in the early‐onset atopy group compared to low atopy (P= 0.01), and a trend for higher cytokine production in the late onset compared to the low atopy cohort was also observed (P= 0.06). T cell responses were similar between early‐ and late‐onset cohorts. Furthermore, a comparison of T cell reactivity between asthmatic and non‐asthmatic individuals revealed significantly higher cytokine production in asthmatics compared to non‐asthmatics (P= 0.02) within both the CR‐allergic and non‐allergic cohorts.Conclusions and clinical relevanceIn conclusion, the present study reports that higher T cell reactivity is associated with allergen sensitization and asthma. Interestingly, no significant difference in T cell reactivity was observed in allergic children with early‐onset versus late‐onset atopy.