Determining the Role of Metal Binding in Protein Cage Assembly
Determining the Role of Metal Binding in Protein Cage Assembly
复制标题
DOI：
10.1007/978-1-4939-2131-7_9
复制
发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Nguyen, Khoa H.
中科院分区：
文献类型：
--
作者：
Grove, Anne;Kushwaha, Ambuj K.;Nguyen, Khoa H.
蛋白质笼的组装可能需要结构金属离子来成核或稳定蛋白质亚基的缔合。我们在这里描述了一种方法来建立金属离子在蛋白质笼组装和稳定性中的作用，重点是检测结构金属离子的存在以及建立低聚状态。描述了用于检测金属的比色测定，沿着用于评估蛋白质笼组装的凝胶过滤测定和用于确定蛋白质稳定性的基于荧光的方法。
Assembly of protein cages may require structural metal ions to nucleate or stabilize association of protein subunits. We describe here an approach to establishing the role of metal ions in protein cage assembly and stability, focusing on detecting the presence of structural metal ions as well as establishing oligomeric state. A colorimetric assay for detection of metal is described, along with a gel filtration assay to assess protein cage assembly and a fluorescence-based method for determining protein stability.