Inhibitory effect of Zn2+ in zinc-containing β-tricalcium phosphate on resorbing activity of mature osteoclasts
Inhibitory effect of Zn2+ in zinc-containing β-tricalcium phosphate on resorbing activity of mature osteoclasts
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DOI：
10.1002/jbm.a.31265
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发表时间：
2008-02-01
影响因子：
4.9
通讯作者：
LeGeros, Racquel Z.
中科院分区：
文献类型：
--
作者：
Yamada, Yasutaka;Ito, Astuo;LeGeros, Racquel Z.
骨-种植体界面因骨吸收而日益不稳定的长期影响是种植体的一个问题，包括生物活性陶瓷。含锌磷酸三钙(ZnTcp)是一种可以克服这一问题的材料。本研究旨在探讨锌离子含量为0.316(316)和0.633(633)的含锌磷酸三钙陶瓷在体外是否抑制成熟破骨细胞的吸收。抑制作用可能是由于凋亡破骨细胞数量的增加和破骨细胞吸收活性的抑制，后者是抑制作用的主要机制。培养24 h后，ZnTCP633组的破骨细胞凋亡率是磷酸三钙陶瓷组的6.3倍。这种凋亡细胞数量的变化对吸收的净贡献比吸收活动的变化小得多。破骨细胞在ZnTcp上形成的肌动蛋白环数比在Tcp上培养的少。破骨细胞中CaII和Cathepsin K/OC2的mRNA表达水平分别为对照组的0.5倍和0.6倍。ZnTCP633的吸收坑体积比TCP633低0.4倍。这些结果提示，锌多糖可抑制成熟破骨细胞的吸收活性，可能是通过局部升高锌离子水平来实现的。从抗骨-种植体界面破骨细胞性骨吸收的角度来看，含锌磷酸三钙的骨替代物或涂层是一种很有前途的生物材料。(C)2007年威利期刊公司。
Lon-term effect of the growing instability of the bone-implant interface due to bone resorption at the interface is a problem for the implants, including bioactive ceramics. Zn2+-containing tricalcium phosphate (ZnTCP) is a material which may overcome this problem. The present study aims to clarify whether Zn2+-containing tricalcium phosphate (ZnTCP) ceramics with a Zn2+ content of 0.316 (ZnTCP316) and 0.633 (ZnTCP633) wt % suppress resorption by mature osteoclasts in vitro. Suppression would be due to an increase in the number of apoptotic osteoclasts and the inhibition of the resorbing activity of osteoclasts, the latter being the major mechanism of the suppression. The number of apoptotic osteoclasts was significantly 6.3 times higher with ZnTCP633 than with tricalcium phosphate ceramic (TCP) after 24-h culture. The net contribution to resorption of this change in apoptotic cell numbers is much smaller than that of the change in resorbing activity. The osteoclasts cultured on ZnTCP formed fewer actin rings than those cultured on the TCP. The mRNA expression of CAII and cathepsin K/OC2 in the osteoclasts on ZnTCP633 was downregulated 0.5-fold and 0.6-fold, respectively, compared with that on the TCP. The volume of resorption pits was downregulated 0.4-fold in the ZnTCP633 than that in TCP. These results suggest that ZnTCPs suppressed the resorbing activity of mature osteoclasts probably through a local increase in the level of Zn2+. Bone substitutes or coating layers containing ZnTCP would be promising biomaterials from the viewpoint of counteracting osteoclastic bone resorption at the bone-implant interface. (c) 2007 Wiley Periodicals, Inc.