INVESTIGATION OF ASSAY OF DOPAMINE USING TRINITROBENZENESULFONIC ACID
INVESTIGATION OF ASSAY OF DOPAMINE USING TRINITROBENZENESULFONIC ACID
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DOI：
10.1016/0003-2697(75)90604-1
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发表时间：
1975-01-01
影响因子：
2.9
通讯作者：
JOHN, R
中科院分区：
文献类型：
--
作者：
CHARTERIS, A;JOHN, R
2,4,6-三硝基苯-1-磺酸与3,4-二羟基-苯基乙胺（多巴胺）之间形成三硝基苯基多巴胺（TNP-多巴胺）的反应很复杂，因为TNP-多巴胺不稳定。反应过程显示，TNP-多巴胺在 20 分钟内升至稳态浓度，约为初始多巴胺浓度的一半，然后在约 1 小时的半衰期内分解。连续萃取到苯中以将 TNP-多巴胺与 TNP-DOPA 分离，这会干扰分光光度测定，也可防止 TNP-多巴胺分解，从而获得定量回收率。该方法已用于令人满意地跟踪多巴脱羧酶催化反应的过程。
The reaction between 2,4,6-trinitrobenzene-1-sulphonic acid with 3,4-dihydroxy-phenylethylamine (dopamine) to form trinitrophenyl dopamine (TNP-dopamine) is found to be complex in that the TNP-dopamine is unstable. The course of the reaction shows TNP-dopamine rising to a steady state concentration equal to about half the initial dopamine concentration within 20 min and then decomposing with a half-time of about 1 hr. Continuous extraction into benzene to separate the TNP-dopamine from TNP-DOPA, which interferes with the spectrophotometric assay, also prevents the decomposition of TNP-dopamine so that quantitative recoveries are obtained. The method has been used to follow the course of a DOPA decarboxylase-catalysed reaction satisfactorily.