Early archives of genetically-restricted proviral DNA in the female genital tract after heterosexual transmission of HIV-1
Early archives of genetically-restricted proviral DNA in the female genital tract after heterosexual transmission of HIV-1
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DOI：
10.1097/qad.0b013e328011f94b
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发表时间：
2007-01-11
期刊：
影响因子：
3.8
通讯作者：
Belec, Laurent
中科院分区：
文献类型：
--
作者：
Chomont, Nicolas;Hocini, Hakim;Belec, Laurent
目的和方法：为了表征通过异性性交在女性中传播的人类免疫缺陷病毒1型（HIV-1）变体，比较了未经治疗的慢性感染和原发感染的非洲女性的血液和宫颈阴道分泌物的配对样品中HIV-1前病毒（DNA）和游离病毒（RNA）的env V1-V3序列。从血浆和生殖器隔室检索的Env RNA序列在原发感染中形成单个簇。相比之下，慢性感染妇女的这两个区室的env RNA序列是不同的。对原发性感染女性的前病毒DNA的分析表明，大多数来自生殖器上皮的HIV-1序列与来自外周血单核细胞的DNA的HIV-1序列以及从血浆和生殖器分泌物中回收的RNA形成独立的簇。同样，对慢性感染妇女生殖器隔室中的前病毒DNA的分析表明，HIV-1的遗传限制簇持续存在。这些观察结果表明，病毒亚群在初次感染早期在女性生殖道中存档为前病毒DNA，并表明在HIV-1的男性向女性传播期间，在女性粘膜部位选择来自男性供体的HIV-1变体。(c)2007年利平科特威廉姆斯&威尔金斯。
Objectives and method: in order to characterize human immunodeficiency virus type 1 (HIV-1) variants that are transmitted in women via heterosexual intercourse, the env V1-V3 sequences of HIV-1 provirus (DNA) and free virus (RNA) in paired samples of blood and cervicovaginal secretions of untreated chronically and primary infected African women were compared.Results: Env RNA sequences retrieved from plasma and genital compartments formed a single cluster in primary infection. In contrast, env RNA sequences from these two compartments were distinct in chronically infected women. Analysis of proviral DNA of primary infected women showed that most HIV-1 sequences derived from the genital epithelia form independent clusters from HIV-1 sequences of DNA from peripheral blood mononuclear cells and RNA recovered from plasma and genital secretions. Similarly, the analysis of proviral DNA in the genital compartment of chronically infected women showed the persistence of genetically-restricted cluster of HIV-1.Conclusions: These observations indicate that a viral subpopulation is archived as proviral DNA in the female genital tract early in primary infection, and suggest that HIV-1 variants from the male donor are selected in the female mucosal site during male to female transmission of HIV-1. (c) 2007 Lippincott Williams & Wilkins.