EFFECTS OF TEMPERATURE ON SOME MEMBRANE CHARACTERISTICS OF CAROTID-BODY CELLS
EFFECTS OF TEMPERATURE ON SOME MEMBRANE CHARACTERISTICS OF CAROTID-BODY CELLS
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DOI：
10.1152/ajpcell.1977.233.1.c35
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发表时间：
1977-01-01
影响因子：
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通讯作者：
EYZAGUIRRE, C
中科院分区：
文献类型：
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作者：
BARON, M;EYZAGUIRRE, C
切除的猫颈动脉体和神经被放置在一个玻璃腔中，盐水在不同的温度下流过。用吸电极记录动作电位。用微电极刺穿Glomus细胞，并通过从微移液管中喷射Procion海军蓝进行鉴定。膜电位（MP）平均为20 mV，输入电阻（R0）为40 ω。冷却诱导去极化，降低R0。这种效应的逆转电位约为-6毫伏。[K+]0不影响MP， R0或冷却效果。缺乏钠会增加MP， R0，并降低冷却效果。低氯化物则有相反的效果。Ca2+和Mg2+对MP和R0有影响，但对温度作用影响不大。瓦巴因降低MP和R0。结果表明，球囊细胞对温度变化非常敏感；MP和R0随温度升高而升高，随温度降低而降低。这些效应主要取决于Cl-和Na+的外部浓度。血管球细胞可能是该组织强烈代谢的部位，因此有助于化学感觉冲动的产生。
Excised cat carotid bodies and nerves were placed in a Lucite chamber through which saline flowed at different temperatures. Action potentials were recorded with a suction electrode. Glomus cells were impaled with microelectrodes and identified by ejecting Procion navy blue from the micropipette. Membrane potentials (MP) averaged 20 mV and input resistances (R0), 40 Momega. Cooling induced depolarization and decreased R0. This effect had a reversal potential of about -6 mV. [K+]0 did not affect MP, R0, or cooling effects. Lack of sodium increased MP, R0, and depressed cooling effects. Low chloride had opposite effects. Ca2+ and Mg2+ influenced MP and R0 but had little effects on temperature actions. Ouabain decreased MP and R0. Results indicate that glomus cells are exquisitely sensitive to temperature changes; their MP and R0 increase with high temperatures and decrease when temperature is lowered. These effects are mostly dependent on the external concentration of Cl- and Na+. Glomus cells may be the site of the intense metabolism of this tissue and thus contribute to the generation of chemosensory impulses.