unc-93(e1500): A behavioral mutant of Caenorhabditis elegans that defines a gene with a wild-type null phenotype.
unc-93(e1500): A behavioral mutant of Caenorhabditis elegans that defines a gene with a wild-type null phenotype.
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unc-93(e1500)：秀丽隐杆线虫的行为突变体，定义了具有野生型无效表型的基因。
DOI：
10.1093/genetics/96.1.147
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
Horvitz,HR
中科院分区：
文献类型：
--
作者：
Greenwald,IS;Horvitz,HR
线虫秀丽隐杆线虫的不协调的产卵缺陷突变unc-93（e1500）III自发地回复到野生型表型。我们描述了102自发和诱变剂诱导的回复突变体，定义了三个位点，两个基因外（sup-9 IIandsup-10 X）和一个基因内。遗传分析表明，ate 1500是一种罕见的可见等位基因，可产生毒性产物，而unc-93基因无效等位基因的产生导致的基因内逆转消除了毒性产物。我们认为unc-93位点的遗传特性，包括e1500突变的自发回复，表明unc-93可能是一个多基因家族的成员。基因外抑制子似乎也是基因活性消除的结果;这些基因可能编码与unc-93基因产物相互作用的调节功能或产物。sunc-93、sup-9和sup-10等基因可用于遗传操作，包括产生缺陷和诱变剂检测。
The uncoordinated, egg-laying-defective mutation,unc-93(e1500) III, of the nematodeCaenorhabditis elegansspontaneously reverts to a wild-type phenotype. We describe 102 spontaneous and mutagen-induced revertants that define three loci, two extragenic (sup-9 IIandsup-10 X) and one intragenic. Genetic analysis suggests thate1500is a rare visible allele that generates a toxic product and that intragenic reversion, resulting from the generation of null alleles of theunc-93gene, eliminates the toxic product. We propose that the genetic properties of theunc-93locus, including the spontaneous reversion of thee1500mutation, indicate thatunc-93may be a member of a multigene family. The extragenic suppressors also appear to arise as the result of elimination of gene activity; these genes may encode regulatory functions or products that interact with theunc-93gene product. Genes such asunc-93, sup-9andsup-10may be useful for genetic manipulations, including the generation of deficiencies and mutagen testing.