Lanthanum(III) Impacts on Metallothionein MTT1 and MTT2 from Tetrahymena thermophila

Lanthanum(III) Impacts on Metallothionein MTT1 and MTT2 from Tetrahymena thermophila
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镧 (III) 对嗜热四膜虫金属硫蛋白 MTT1 和 MTT2 的影响

DOI:
10.1007/s12011-011-9004-2
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发表时间:
2011-02
影响因子:
3.9
通讯作者:
Wang, Wei
Wang, Wei
中科院分区:
生物学3区
文献类型:
--
作者:
Wang, Qinglu;Xu, Jing;Zhu, Yajing;Chai, Baofeng;Liang, Aihua;Wang, Wei

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Metallothionein MTT1 and MTT2 from Tetrahymena thermophila are sulfydryl-rich proteins that can bind to and are inducible by heavy metals such as mercury, cadmium, zinc, and copper. However, little is known about the induction and binding of T. thermophila metallothionein by trivalent metals. In this study, we found that 10-80 μM La(3+) can promote Tetrahymena cells proliferation, and fluorescence spectrum analysis showed that La(3+) can enter T. thermophila cells. Real-time quantitative polymerase chain reaction showed La(3+) induced the expression of MTT1 and MTT2. Furthermore, Fluorescence analysis indicated La(3+) bind to MTT1 and MTT2. These results implied that La(3+) could interact with MTT1 and MTT2 via aspartic or glutamic acid oxygen atoms.
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