Ptychographic microscope for three-dimensional imaging
Ptychographic microscope for three-dimensional imaging
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DOI：
10.1364/oe.22.012513
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发表时间：
2014-05-19
期刊：
影响因子：
3.8
通讯作者：
Maiden, A. M.
中科院分区：
文献类型：
--
作者：
Godden, T. M.;Suman, R.;Maiden, A. M.
层析是一种相干成像技术，能够从一组衍射图案生成样品的图像。这项技术的一个局限性是假设照明相干光束和样品之间存在乘性相互作用，这使得在微米级可见光成像的情况下，光刻只限于厚度不超过几十微米的样品。通过将样品分成轴向切片，我们在最近的工作中证明了这种厚度限制可以放松，更重要的是，可以使用单一的层析数据集实现粗略的光学切片。在这里，我们将我们的技术应用于从改进的光学显微镜收集的数据，以实现对厚度达150米的样品的轴向光学切片深度减少到2米。此外，我们将成像的截面数量从以前的5个增加到这里的34个。我们的结果与从共聚焦显微镜收集的切片图像相比很好，但具有相位对比度强的额外优势，这消除了对样品染色的需要。(C)2014年美国光学学会
Ptychography is a coherent imaging technique that enables an image of a specimen to be generated from a set of diffraction patterns. One limitation of the technique is the assumption of a multiplicative interaction between the illuminating coherent beam and the specimen, which restricts ptychography to samples no thicker than a few tens of micrometers in the case of visible-light imaging at micron-scale resolution. By splitting a sample into axial sections, we demonstrated in recent work that this thickness restriction can be relaxed and whats-more, that coarse optical sectioning can be realized using a single ptychographic data set. Here we apply our technique to data collected from a modified optical microscope to realize a reduction in the optical sectioning depth to 2 m in the axial direction for samples up to 150 m thick. Furthermore, we increase the number of sections that are imaged from 5 in our previous work to 34 here. Our results compare well with sectioned images collected from a confocal microscope but have the added advantage of strong phase contrast, which removes the need for sample staining. (C) 2014 Optical Society of America