Identification of genes predicting unfavorable prognosis in hepatitis B virus-associated hepatocellular carcinoma.
Identification of genes predicting unfavorable prognosis in hepatitis B virus-associated hepatocellular carcinoma.
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乙型肝炎病毒相关性肝细胞癌预测不良预后基因的鉴定
DOI：
10.21037/atm-21-2085
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发表时间：
2021-06
影响因子：
--
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Sha M;Cao J;Zong ZP;Xu N;Zhang JJ;Tong Y;Xia Q
背景：寻找预测B型肝炎病毒（HBV）相关肝细胞癌（HCC）预后不良的潜在关键基因。方法从GEO数据库中获得GSE 121248、GSE 62232和GSE 55092的基因表达谱，并进行分析。应用Limma软件包和Venn图软件筛选HBV相关性肝癌组织与癌旁正常组织的差异表达基因。通过基因本体论（GO）和京都基因和基因组百科全书（KEGG）进行DEG的功能评估。通过蛋白质-蛋白质相互作用（PPI）网络选择Hub基因，并通过GSE 14520临床数据进一步验证。结果通过对3个数据库的分析，共发现26个上调基因和76个下调基因。GO和KEGG分析表明，这些基因参与细胞分裂，代谢相关的生物学过程，p53通路和细胞周期等。PPI网络显示14个hub DEG（TOP 2A、HMMR、DTL、CCNB 1、NEK 2、PBK、RACGAP 1、PRC 1、CDK 1、RRM 2、ECT 2、BUB 1B、ANLN和ASPM）表达异常最严重，并有可能区分HBV相关的HCC和非癌组织。对hub基因的进一步生存分析表明，TOP 2A的高表达与HBV相关HCC的不良临床结局显著相关。结论TOP 2A基因可能是HBV相关性肝癌预后的关键基因，并可作为HBV相关性肝癌治疗的靶点。
Background To identify potential key genes predicting unfavorable prognosis in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC). Methods Gene expression profiles of GSE121248, GSE62232, and GSE55092 from the GEO database were obtained and analyzed. Differentially expressed genes (DEGs) between HBV-associated HCC tissues and adjacent normal tissues were screened by the limma package and Venn diagram software. Functional assessment of DEGs was performed by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). Hub genes were selected by the protein-protein interaction (PPI) network and further validated by GSE14520 clinical data. Results A total of 26 up-regulated genes and 76 down-regulated genes were identified by analyzing three databases. GO and KEGG analysis demonstrated that these genes were involved in cell division, metabolism-related biological processes, the p53 pathway, and the cell cycle, among others. PPI network suggested that 14 hub DEGs (TOP2A, HMMR, DTL, CCNB1, NEK2, PBK, RACGAP1, PRC1, CDK1, RRM2, ECT2, BUB1B, ANLN, and ASPM) were most dysregulated and had potential to distinguish between HBV-associated HCC and noncancerous tissues. Further survival analysis of hub genes demonstrated that high expression of TOP2A was significantly associated with poor clinical outcomes of HBV-associated HCC. Conclusions TOP2A might serve as a key gene for prognosis and as a therapeutic target for HBV-associated HCC.
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影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
影响因子：
25.7
作者：
Fujiwara N;Friedman SL;Goossens N;Hoshida Y
通讯作者：
Hoshida Y
乙型肝炎病毒相关肝细胞癌关键生物标志物和通路的综合研究
影响因子：
3.9
作者：
Liao, Xiwen;Yu, Tingdong;Peng, Tao
通讯作者：
Peng, Tao
DOI：
10.1158/1078-0432.ccr-17-0413
发表时间：
2017-11-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Labbé DP;Sweeney CJ;Brown M;Galbo P;Rosario S;Wadosky KM;Ku SY;Sjöström M;Alshalalfa M;Erho N;Davicioni E;Karnes RJ;Schaeffer EM;Jenkins RB;Den RB;Ross AE;Bowden M;Huang Y;Gray KP;Feng FY;Spratt DE;Goodrich DW;Eng KH;Ellis L
通讯作者：
Ellis L
影响因子：
1.6
作者：
Wu, Min;Liu, Zhaobo;Li, Ning
通讯作者：
Li, Ning