Carried meningococci in the Czech Republic: A diverse recombining population
Carried meningococci in the Czech Republic: A diverse recombining population
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DOI：
10.1128/jcm.38.12.4492-4498.2000
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发表时间：
2000-12-01
影响因子：
9.4
通讯作者：
Maiden, MCJ
中科院分区：
文献类型：
--
作者：
Jolley, KA;Kalmusova, J;Maiden, MCJ
病原细菌的种群和进化分析经常受到集中于侵袭性疾病患者的分离样本策略的阻碍。这对于革兰氏阴性脑膜炎奈球菌尤其如此，这是全世界败血症和脑膜炎的一种病因。脑膜炎球菌分离菌收集物几乎完全包括来自侵袭性脑膜炎球菌病患者的生物体，尽管这种细菌是人类鼻咽部的共生菌，很少引起病理影响。在本研究中，基于分子生物学的技术用于建立1993年期间从捷克共和国健康年轻人分离的156种脑膜炎球菌的遗传关系。这些样本中没有一个人与患有侵袭性疾病的病人有已知的联系。多位点序列分型（Multilocus sequence typing， MLST）表明，该菌群具有高度的多样性，包括71个不同的序列类型（sequence types, STs），分别归属于34个不同的复合体或谱系。存在三个先前确定的高侵袭性谱系：26株（17%）属于ST-41复合体（谱系3）；4个（2.6%）属于ST-11（电泳型[ET-37]）复合体，1个（0.6%）属于ST-32 （ET-5）复合体。这些数据与大多数核苷酸序列多样性是由于等位基因通过水平遗传交换而重新组合的观点一致。
Population and evolutionary analyses of pathogenic bacteria are frequently hindered by sampling strategies that concentrate an isolates from patients dth invasive disease. This is especially so for the gram-negative diplococcus Neirseria meningitidis, a cause of septicemia and meningitis worldwide. Meningococcal isolate collections almost exclusively comprise organisms originating from patients with invasive meningococcal disease, although this bacterium is a commensal inhabitant of the human nasopharynx and very rarely causes pathological effects. In the present study, molecular biology-based techniques were used to establish the genetic relationships of 156 meningococci isolated from healthy young adults in the Czech Republic during 1993. None of the individuals sampled had known links to patients with invasive disease. Multilocus sequence typing (MLST) showed that the bacterial population was highly diverse, comprising 71 different sequence types (STs) which were assigned to 34 distinct complexes or lineages. Three previously identified hyperinvasive lineages were present: 26 isolates (17%) belonged to the ST-41 complex (lineage 3); 4 (2.6%) belonged to the ST-11 (electrophoretic type [ET-37]) complex, and 1 (0.6%) belonged to the ST-32 (ET-5) complex. The data were consistent with the view that most nucleotide sequence diversity resulted from the reassortment of alleles by horizontal genetic exchange.