Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage.
Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage.
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肽/组氨酸转运蛋白 SLC15A3 在 Toll 样受体介导的巨噬细胞炎症反应中的调节和生物学作用
DOI：
10.1038/s41419-018-0809-1
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发表时间：
2018-07-10
影响因子：
9
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Song F;Yi Y;Li C;Hu Y;Wang J;Smith DE;Jiang H
The peptide/histidine transporter SLC15A3 is responsible for transporting histidine, certain dipeptide and peptidomimetics from inside the lysosome to cytosol. Previous studies have indicated that SLC15A3 transcripts are mainly expressed in the lymphatic system, however, its regulation and biological role in innate immune responses and inflammatory diseases are as yet unknown. In this study, mouse peritoneal macrophages (PMs), mouse bone marrow-derived macrophages (BMDMs), the human acute monocytic leukemia cell line THP-1 and the human lung epithelial carcinoma cell line A549 were used to investigate the regulation and biological role of SLC15A3 in TLR-mediated inflammatory responses. Our results showed that SLC15A3 was upregulated by TLR2, TLR4, TLR7 and TLR9 ligands in macrophages at both the mRNA and protein levels via activation of NF-κB (nuclear factor-kappa-B), MAPK (mitogen-activated protein kinase) and IRF3 (interferon regulatory factor 3). Furthermore, knockdown or overexpression of SLC15A3 influenced the TLR4-triggered expression of proinflammatory cytokines. A reporter gene assay showed that the SLC15A3 promotor contained potential NF-κB binding sites, which were reasonable for regulating SLC15A3 by TLR-activation through NF-κB signaling. Additionally, SLC15A3 expression was increased and positively related to inflammation in mice with bacterial peritonitis. The collective findings suggest that SLC15A3 is regulated by various TLRs, and that it plays an important role in regulating TLR4-mediated inflammatory responses.
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影响因子：
8.7
作者：
Hamerman JA;Pottle J;Ni M;He Y;Zhang ZY;Buckner JH
通讯作者：
Buckner JH
DOI：
10.1016/j.bbagen.2013.12.026
发表时间：
2014-06-01
影响因子：
3
作者：
Geillinger, Kerstin E.;Kipp, Anna P.;Daniel, Hannelore
通讯作者：
Daniel, Hannelore
影响因子：
4.4
作者：
Das A;Chai JC;Kim SH;Lee YS;Park KS;Jung KH;Chai YG
通讯作者：
Chai YG
DOI：
10.4049/jimmunol.1402312
发表时间：
2015-05-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Guo H;Zhang J;Zhang X;Wang Y;Yu H;Yin X;Li J;Du P;Plumas J;Chaperot L;Chen J;Su L;Liu Y;Zhang L
通讯作者：
Zhang L
DOI：
10.1152/ajpgi.00171.2006
发表时间：
2006-11-01
影响因子：
4.5
作者：
Shimakura, Jin;Terada, Tomohiro;Inui, Ken-ichi
通讯作者：
Inui, Ken-ichi