Identification and comparative analysis of aluminum-induced microRNAs conferring plant tolerance to aluminum stress in soybean
Identification and comparative analysis of aluminum-induced microRNAs conferring plant tolerance to aluminum stress in soybean
复制标题
铝诱导大豆耐受铝胁迫的 microRNA 的鉴定和比较分析
DOI：
10.1007/s10535-017-0752-5
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发表时间：
2018-01-01
影响因子：
1.5
通讯作者：
Yang, Y. H.
中科院分区：
文献类型：
--
作者：
Huang, S. C.;Lu, G. H.;Yang, Y. H.
酸性土壤中铝（Al）的毒性是限制作物生产的主要因素。尽管铝反应的分子机制已被广泛研究，但 microRNA (miRNA) 介导的不同大豆基因型的铝耐受差异仍然很大程度上未知。在本研究中，两种大豆[Glycine max (L.) Merr.]基因型，耐铝BX10和铝敏感BD2，分别用0和50 mu M AlCl3处理，然后用于构建用于深度测序的miRNA文库。结果显示 453 个 miRNA 的表达模式受到铝胁迫的影响。我们还鉴定了 32 个差异表达的 miRNA：19 个在 BX10 中，7 个在 BD2 中，6 个在两种基因型中。对假定目标基因的基因本体分析表明，应激反应基因和氨基酸代谢相关过程优先存在于BX10中。综合分析表明，保守的 miRNA，如 gma-miR166k/o、gma-miR390g 和 gma-miR396c/k，介导 BX10 中的根伸长，而 gma-miR169r 则触发 BD2 中的氧化应激。这些过程可以被视为赋予 BX10 和 BD2 差异铝耐受性的重要机制。这项研究为不同大豆基因型的不同铝反应机制提供了新的见解。
Aluminum (Al) toxicity in acidic soils is a major factor restricting crop production. Although the molecular mechanisms of Al responses have been extensively investigated, microRNA (miRNA) mediated differential Al tolerance in different soybean genotypes remains largely unknown. In this study, two soybean [Glycine max (L.) Merr.] genotypes, Al-tolerant BX10 and Al-sensitive BD2, were treated with 0 and 50 mu M AlCl3 and then used to construct the miRNA libraries for deep sequencing. Results revealed 453 miRNAs, whose expression patterns were affected by Al stress. We also identified 32 differentially expressed miRNAs: 19 in BX10, 7 in BD2, and 6 in both genotypes. The gene ontology analysis of their putative target genes indicated that stress-responsive genes and amino-acid-metabolism-related processes preferentially existed in BX10. Comprehensive analysis demonstrated that conserved miRNAs, such as gma-miR166k/o, gma-miR390g, and gma-miR396c/k, mediated root elongation in BX10, whereas gma-miR169r triggered oxidative stress in BD2. These processes could be regarded as important mechanisms conferring differential Al tolerance in BX10 and BD2. This study provided new insights into different Al response mechanisms in various soybean genotypes.