Biodegradation behaviors of magnesium(Mg)-based alloy nails in autologous bone grafts: In vivo study in rabbit skulls
Biodegradation behaviors of magnesium(Mg)-based alloy nails in autologous bone grafts: In vivo study in rabbit skulls
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DOI：
10.1177/22808000221095230
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发表时间：
2022-01
影响因子：
2.5
通讯作者：
Yuta Yanagisawa;Y. Shimizu;T. Mukai;Yuya Sano;Kenji Odashima;N. Ikeo;H. Saito;K. Yamauchi;Tetsu Takahashi;H. Kumamoto
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文献类型：
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作者：
Yuta Yanagisawa;Y. Shimizu;T. Mukai;Yuya Sano;Kenji Odashima;N. Ikeo;H. Saito;K. Yamauchi;Tetsu Takahashi;H. Kumamoto
目的：在这项研究中，使用镁锌钙三元合金制成的骨固定钉进行自体骨移植，使用兔颅骨。材料与方法：制备两种类型的骨内固定钉：2.5mm宽，3mm长和2.5mm宽，2mm长。从顶骨切除直径为5 mm的盘状骨，并用3 mm长的髓内钉固定。作为对照组，将2 mm长的髓内钉打入现有骨中。术后1、4、12和24周处死兔子。显微X线CT观察标本的形态，甲基丙烯酸甲酯包埋标本，制备不脱钙标本。使用能量色散X射线光谱（EDS）检查元素的体内定位。结果：显微X线CT图像显示，由于骨移植物和对照组的降解，样品的体积减少。未观察到两组之间的降解量存在显著差异，但在每组中均观察到特征性降解过程。用茜素红S染色的样品在指甲周围显示出无定形区域，这被认为是腐蚀产物，并与新形成的骨直接接触。EDS分析表明，腐蚀初期腐蚀产物主要由镁和氧组成，而长期观察时在表层检测到钙和磷。结论：镁合金髓内钉的降解速度取决于髓内钉的形状和周围组织条件。镁合金髓内钉表面形成一层磷酸钙层，说明髓内钉的降解速度较慢。
Objective: In this study, autologous bone grafts using bone-fixing nails made of magnesium-zinc-calcium ternary alloys were performed using rabbit skulls. Material and methods: Two types of nails for bone fixation were prepared: 2.5 mm width, 3 mm length and 2.5 mm width, 2 mm length. A disk-shaped bone with a diameter of 5 mm was resected from the parietal bone and fixed with a 3 mm long nail. As a control group, a 2 mm long nail was driven into the existing bone. The rabbits were sacrificed at 1, 4, 12, and 24 weeks after surgery. The resected samples were observed with micro X-ray CT, and embedded in methyl methacrylate to prepare non-decalcified specimens. The in vivo localization of elements was examined using energy-dispersive X-ray spectroscopy (EDS). Results: Micro X-ray CT images of samples showed volume reduction due to degradation in both the bone graft and control groups. No significant difference in the amount of degradation between the two groups was observed, however characteristic degradation processes were observed in each group. The samples stained with alizarin red S showed amorphous areas around the nails, which were considered as corrosion products and contacted directly with the newly formed bones. EDS analysis showed that corrosion products were mainly composed of magnesium and oxygen at an early stage, while calcium and phosphorus were detected on the surface layer during the long-term observation. Conclusions: The degradation speed of the magnesium alloy nails varied depending on the shapes of the nails and surrounding tissue conditions. A calcium phosphate layer was formed on the surface of magnesium alloy nails, suggesting that the degradation rate of the nail was slow.