Long noncoding RNA H19 inhibits the proliferation of fetal liver cells and the Wnt signaling pathway
Long noncoding RNA H19 inhibits the proliferation of fetal liver cells and the Wnt signaling pathway
复制标题
长链非编码RNA H19抑制胎儿肝细胞增殖及Wnt信号通路
DOI：
10.1002/1873-3468.12078
复制
发表时间：
2016-02-01
期刊：
影响因子：
3.5
通讯作者：
Wang, Fang
中科院分区：
文献类型：
--
作者：
Wang, Shaobing;Wu, Xia;Wang, Fang
在本研究中，我们发现H19是肝脏发育过程中差异表达最强烈的长链非编码RNA （lncRNA）。H19可能通过阻断异质核核糖核蛋白（hnRNP） U与肌动蛋白的相互作用而抑制胎儿肝细胞的增殖，从而导致基因转录抑制。基于ChIP - seq分析，我们发现hnRNP U - binding基因中富集了Wnt信号通路相关基因。进一步的研究表明，hnRNP U对细胞增殖和Wnt/β - catenin信号通路活性的影响与H19相反，hnRNP U在这一过程中起着非常重要的作用。
In this study, we found that H19 is the most strongly differentially expressed long noncoding RNA (lncRNA) during liver development. H19 may inhibit the proliferation of fetal liver cells by blocking the interaction between heterogeneous nuclear ribonucleoprotein (hnRNP) U and actin, which results in gene transcriptional repression. Based on ChIP‐seq analysis, we found that genes involved in the Wnt signaling pathway are enriched among hnRNP U‐binding genes. Further investigation demonstrated that hnRNP U has opposing effects on cell proliferation and Wnt/β‐catenin signaling pathway activity compared to H19 and that hnRNP U is very important in this process.