An Efficient Procedure for Removal and Inactivation of Alpha-Synuclein Assemblies from Laboratory Materials.
An Efficient Procedure for Removal and Inactivation of Alpha-Synuclein Assemblies from Laboratory Materials.
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DOI：
10.3233/jpd-150691
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Melki R
中科院分区：
文献类型：
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作者：
Bousset L;Brundin P;Böckmann A;Meier B;Melki R
背景：预形成的 α-突触核蛋白原纤维在培养细胞和体内培养可溶性 α-突触核蛋白的聚集。这一发现和其他发现引发了这样的观点：α-突触核蛋白原纤维具有类似朊病毒的特性。目的：由于 α-突触核蛋白原纤维不应被视为无害，因此需要对实验室工作台和非一次性实验室材料进行净化和灭活程序。方法：我们评估了旨在分解 α-突触核蛋白原纤维并降低其传染性的不同程序的有效性。我们检查了不同的市售清洁剂，以去除吸附在非一次性材料和实验室中最常见的材料（例如塑料、玻璃、铝或不锈钢表面）上的 α-突触核蛋白组件。结果：我们表明，旨在降低 PrP 朊病毒感染性的方法既不能有效去除吸附在实验室常用的不同材料上的 α-突触核蛋白组装体，也不能有效地分解蛋白质的纤维形式。相比之下，商业去污剂和 SDS 都能将 α-突触核蛋白组件从污染的表面上分离出来，并分解原纤维。结论：我们描述了三种有效去除和分解 α-突触核蛋白种子的清洁程序。这些方法依赖于使用与实验室中大多数非一次性工具兼容的清洁剂。该程序易于实施，并显着降低与处理 α-突触核蛋白组件相关的任何潜在风险。
Background: Preformed α-synuclein fibrils seed the aggregation of soluble α-synuclein in cultured cells and in vivo. This, and other findings, has kindled the idea that α-synuclein fibrils possess prion-like properties. Objective: As α-synuclein fibrils should not be considered as innocuous, there is a need for decontamination and inactivation procedures for laboratory benches and non-disposable laboratory material. Methods: We assessed the effectiveness of different procedures designed to disassemble α-synuclein fibrils and reduce their infectivity. We examined different commercially available detergents to remove α-synuclein assemblies adsorbed on materials that are not disposable and that are most found in laboratories (e.g. plastic, glass, aluminum or stainless steel surfaces). Results: We show that methods designed to decrease PrP prion infectivity neither effectively remove α-synuclein assemblies adsorbed to different materials commonly used in the laboratory nor disassemble the fibrillar form of the protein with efficiency. In contrast, both commercial detergents and SDS detached α-synuclein assemblies from contaminated surfaces and disassembled the fibrils. Conclusions: We describe three cleaning procedures that effectively remove and disassemble α-synuclein seeds. The methods rely on the use of detergents that are compatible with most non-disposable tools in a laboratory. The procedures are easy to implement and significantly decrease any potential risks associated to handling α-synuclein assemblies.