An evaluation of the effects of culture medium osmolality and pH on metabolic cooperation between Chinese hamster V79 cells.
An evaluation of the effects of culture medium osmolality and pH on metabolic cooperation between Chinese hamster V79 cells.
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培养基渗透压和 pH 对中国仓鼠 V79 细胞代谢协同影响的评价。
DOI：
10.1093/carcin/8.9.1257
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发表时间：
1987
期刊：
影响因子：
4.7
通讯作者：
M. E. Volpenhein
中科院分区：
文献类型：
--
作者：
R. L. Binder;M. E. Volpenhein
中国仓鼠V79细胞代谢协作试验间接测量通过间隙连接的细胞间通信的抑制，并且可以检测许多肿瘤促进剂。此处描述的实验旨在确定培养基渗透压摩尔浓度和pH值是否影响本试验的终点，因为高渗溶液和细胞质酸化可解偶联其他系统中的间隙连接。培养基中高渗浓度的蔗糖和甘露醇确实抑制了代谢合作，但这些溶质在不同的渗透压范围内具有活性。在类似的实验中，氯化钠、氯化胆碱和葡萄糖醛酸钠对代谢合作没有影响。此外，获得的数据表明，渗透作用不太可能有助于抑制代谢合作的糖精钠。它的结论是，高渗培养条件下不一定会导致抑制代谢合作，和添加的溶质的属性是重要的，在确定所观察到的效果。此外，发现在供试品正常存在期间，将培养基pH值缓冲在约6.6和7.7之间对代谢合作没有影响。
The Chinese hamster V79 cell metabolic cooperation assay indirectly measures an inhibition of intercellular communication through gap junctions and can detect many tumor promoters. The experiments described here were designed to determine whether culture medium osmolality and pH affect the endpoint of this assay, since hypertonic solutions and cytoplasmic acidification can uncouple gap junctions in other systems. Hypertonic concentrations of sucrose and mannitol in medium did inhibit metabolic cooperation, but these solutes were active over different ranges of osmolalities. In similar experiments, sodium chloride, choline chloride and sodium glucuronate had no effect on metabolic cooperation. Furthermore, data were obtained indicating that osmotic effects are not likely to contribute to the inhibition of metabolic cooperation by sodium saccharin. It is concluded that hypertonic culture conditions do not necessarily cause an inhibition of metabolic cooperation, and the properties of an added solute are important in determining the effects observed. Additionally, it was found that buffering the culture medium pH between approximately 6.6 and 7.7, during the period when test substances are normally present, had no effect on metabolic cooperation.