Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii
Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii
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DOI：
10.1111/j.1365-313x.2008.03767.x
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发表时间：
2009-04-01
期刊：
影响因子：
7.2
通讯作者：
Baulcombe, David
中科院分区：
文献类型：
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作者：
Molnar, Attila;Bassett, Andrew;Baulcombe, David
MicroRNA（miRNAs）是一类长度为21 ~ 22个核苷酸的小分子RNA，具有重要的调控作用。它们由具有不完全匹配的折返区域的较长RNA分子加工而成，并且它们在调节mRNA的稳定性和翻译中起作用。最近，我们和其他人已经证明，单细胞的莱茵衣藻，像不同的多细胞生物体，包含miRNA。这些RNA类似于陆地植物的miRNA，因为它们指导具有miRNA互补基序的靶mRNA的位点特异性切割，并且可能在生长和发育中充当调节分子。利用这些发现，我们开发了一种新的人工miRNA系统的基础上连接的DNA寡核苷酸，可用于特定的高通量基因沉默在绿色藻类。
MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.