Highly expressed genes in gonads of the bat star Patiria miniata: gene ontology, expression differences, and gamete recognition loci
Highly expressed genes in gonads of the bat star Patiria miniata: gene ontology, expression differences, and gamete recognition loci
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DOI：
10.1111/ivb.12029
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发表时间：
2013-09-01
影响因子：
1.2
通讯作者：
Foster, Adam
中科院分区：
文献类型：
--
作者：
Hart, Michael W.;Foster, Adam
在广播产卵的海洋动物和植物的交配系统的进化分析集中在精子和卵子表面蛋白和糖肽介导的生殖相互作用在不同阶段的配子识别。要更好地理解这种相互作用的生态学和进化，就需要将我们的知识扩展到在精子和卵子中表达的多个基因，这些基因来自不同的分类群体，具有不同的受精模式。在这里，我们使用易于访问的下一代测序方法和桌面生物信息学的特点，在睾丸和卵巢的asterinid海星星Patiria miniata，包括男性和女性表达的分子的基因本体注释，并描述了两个基因，编码参与受精卵表面分子，以前没有研究海星的剧目。结果被用来对比睾丸和卵巢之间的表达差异，并制定配子特异性表达的假设。我们还探讨了卵巢基因表达的差异之间的多个女性从北方和南方的人口，显示在许多非表达位点的核苷酸分化，并在配子识别位点。
Evolutionary analysis of mating systems in broadcast-spawning marine animals and plants has focused on sperm- and egg-surface proteins and glycopeptides that mediate reproductive interactions at different stages of gamete recognition. Improved understanding of the ecology and evolution of such interactions depends on extending our knowledge to multiple genes expressed in both sperm and eggs of diverse taxonomic groups with different modes of fertilization. Here, we use readily accessible next-generation sequencing methods and desktop bioinformatics to characterize the repertoire of highly expressed genes in testes and ovaries of the asterinid sea star Patiria miniata, including gene ontology annotations for male- and female-expressed molecules, and descriptions of two genes that encode egg-surface molecules involved in fertilization that have not previously been studied in sea stars. The results are used to contrast expression differences between the testis and ovary, and to develop hypotheses of gamete-specific expression. We also explore differences in ovary gene expression among multiple females from northern and southern populations that show nucleotide differentiation at many non-expressed loci and at a gamete recognition locus.