Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ‘Morita navel’ orange (Citrus sinensis Osbeck)
Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ‘Morita navel’ orange (Citrus sinensis Osbeck)
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DOI：
10.1007/s00299-004-0777-z
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发表时间：
2004-03
影响因子：
6.2
通讯作者：
K. Takami;A. Matsumara;M. Yahata;T. Imayama;H. Kunitake;H. Komatsu
中科院分区：
文献类型：
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作者：
K. Takami;A. Matsumara;M. Yahata;T. Imayama;H. Kunitake;H. Komatsu
利用电融合技术获得了圆叶金柑和森田脐橙橙子胚性愈伤组织原生质体的属间体细胞杂种。在获得的八种不同融合株中，六种表现出正常形态，而其余两种表现出畸形。所有再生植株的叶片形态均为中间型，叶片厚而圆，具有多倍体的典型特征。经流式细胞仪倍性分析和根尖细胞染色体计数，确定为双二倍体（2n=4×=36）。通过随机扩增多态性DNA和切割扩增多态性序列（CAPS）分析证实融合产物的杂交性。此外，叶绿体（cp）和线粒体（mt）DNA的CAPS分析表明，这些体细胞杂种含有圆枇杷的cp-和mt-DNA没有重组的区域分析。
Intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (Fortunella japonicaSwingle) and ‘Morita navel’ orange (Citrus sinensisOsbeck) were produced by electrofusion. Among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. All the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. Ploidy analyses by flow cytometry and chromosome counting in root-tip cells revealed that these plants are amphidiploid (2n=4×=36). Hybridity of the fusion products was confirmed by random amplified polymorphic DNA and cleaved amplified polymorphic sequence (CAPS) analyses. Furthermore, analyses of chloroplast (cp) and mitochondrial (mt) DNA by CAPS showed that these somatic hybrids contained cp- and mt-DNA of round kumquat without recombination in the regions analyzed.