Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging.
Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging.
复制标题
用于细胞成像的变构荧光 RNA 传感器的合理设计。
DOI：
10.1007/978-1-0716-1499-0_11
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
You,Mingxu
中科院分区：
文献类型：
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作者：
Yu,Qikun;Zheng,Ru;Narayanan,Manojkumar;You,Mingxu
基于荧光的工具在研究细胞功能方面是无价的。传统的基于小分子或蛋白质的荧光传感器已广泛用于细胞成像，但其靶点的选择仍然有限。近年来，基于荧光rna的传感器受到了广泛的关注。这种新型的传感器系统可以作为一个通用的平台来检测各种细胞目标。在这里，我们描述了合理设计、优化和应用基于荧光rna的传感器的步骤，利用四环素在活体细胞内的成像。以科利塞尔为例。
Fluorescence-based tools are invaluable in studying cellular functions. Traditional small molecule or protein-based fluorescent sensors have been widely used for the cellular imaging, but the choice of targets is still limited. Recently, fluorogenic RNA-based sensors gained lots of attention. This novel sensor system can function as a general platform for various cellular targets. Here, we describe the steps to rationally design, optimize, and apply fluorogenic RNA-based sensors, using the intracellular imaging of tetracycline in livingE. colicells as an example.