Amino-terminal location of pyridinoline in dentin collagen.
Amino-terminal location of pyridinoline in dentin collagen.
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吡啶啉在牙本质胶原蛋白中的氨基末端位置。
作者：
Y. Kuboki;M. Okuguchi;H. Takita;M. Kimura;M. Tsuzaki;A. Takakura;S. Tsunazawa;F. Sakiyama;H. Hirano
交联被认为是表征牙本质和骨胶原的可钙化性的主要因素之一。构成牙本质胶原的主要交联的脱氢-二羟基赖氨酸正亮氨酸和吡啶啉迄今为止仅位于分子的羧基末端肽[α 1（I）-链87 x α 1（I）-链16 C]。这种情况表明，氨基端肽部分可能是“开放的”，没有分子间交联的硬组织胶原纤维。然而，本研究提供的证据表明，吡啶啉也位于氨基端肽（α 1-链9 N或α 2-链5 N）和α 1-链930。用胰蛋白酶消化牛牙本质胶原，然后在消化前后在60 ℃下加热。该方法可获得完整的牙本质胶原蛋白胰蛋白酶肽段。用Sephadex G-50超微细柱、DEAE-纤维素柱和反相HPLC分离了小分子的吡啶啉类荧光肽。几种分离的肽的自动Edman分析揭示了五个残基序列，Gly-Ile-X-Gly-His-Arg，其唯一的分配是α 1-链928-933。结合氨基酸组成分析，认为吡啶啉不仅存在于牙本质胶原的羧基端，而且存在于氨基端。
Cross-linking is believed to be one of the major factors that characterize the calcifiability of dentin and bone collagens. Dehydro-dihydroxylysinonorleucine and pyridinoline which constitute the principal cross-links of dentin collagen have so far been located only in the carboxy terminal telopeptide of the molecules [alpha 1(I)-chain 87 x alpha 1(I)-chain 16C]. This situation suggested that the amino terminal telopeptide portion might be "open" without intermolecular cross-linking in hard tissue collagen fibrils. However, the present study provided evidence that pyridinoline is also located in amino-terminal telopeptides (alpha 1-chain 9N or alpha 2-chain 5N) and alpha 1-chain 930. Bovine dentin collagen was digested with trypsin followed by heating at 60 degrees C before and after the digestion. This method gave complete trypsin peptides of dentin collagen. Fluorescent pyridinoline peptides with a smaller molecular size were isolated by Sephadex G-50 superfine, DEAE-cellulose and reverse-phase HPLC. Automatic Edman analysis of several isolated peptides revealed the five-residue sequence, Gly-Ile-X-Gly-His-Arg, the only assignment of which was alpha 1-chain 928-933. The above evidence together with the amino acid compositions of the peptides led to the conclusion that pyridinoline is located not only in the carboxy-terminal but also in the amino-terminal telopeptide in dentin collagen.
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DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yamauchi,M;London,RE;Guenat,C;Hashimoto,F;Mechanic,GL
通讯作者：
Mechanic,GL
影响因子：
2.9
作者：
M. Yamauchi;E. P. Katz;E. P. Katz;G. Mechanic
通讯作者：
M. Yamauchi;E. P. Katz;E. P. Katz;G. Mechanic
牛关节软骨 II 型胶原中羟基吡啶鎓交联位点的鉴定。
影响因子：
2.9
作者：
Wu,JJ;Eyre,DR
通讯作者：
Eyre,DR
胶原蛋白中基于组氨酸的天然亚胺交联及其位置。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bernstein,PH;Mechanic,GL
通讯作者：
Mechanic,GL